Moon Jeong Mi, Lee Song Eun, Min Yong Il, Jung Chaeyong, Ahn Kyu Youn, Nam Kwang Il
Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University, Gwangju, Korea.
Anat Cell Biol. 2011 Jun;44(2):98-105. doi: 10.5115/acb.2011.44.2.98. Epub 2011 Jun 30.
To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. The results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
为了鉴定参与流产过程的基因，将正常妊娠子宫与脂多糖（LPS）诱导流产的子宫进行比较。在妊娠第6天，在不同时间点用LPS处理小鼠以诱导流产。将总RNA应用于cDNA微阵列以分析表达改变的基因。在LPS诱导流产的早期阶段（2小时），上调的基因主要由免疫反应基因组成，包括Ccl4、Ccl2、Cxcl13、Gbp3、Gbp2、Mx2、H2-Eb1、Irf1和Ifi203。与Toll样受体信号传导相关的基因也过表达。在流产后期（12 - 24小时），许多基因被抑制而非激活，这些基因主要与细胞外基质、细胞骨架和抗凋亡相关。通过实时逆转录 - 聚合酶链反应证实了几个选定基因的表达改变。结果表明，在LPS处理的妊娠子宫中许多已知基因发生了改变，这意味着参与LPS诱导流产的基因的分子机制是复杂的。对这种表达谱的进一步分析将有助于我们理解流产的病理生理基础。
