Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Chaoyang, Beijing 100026, China.
Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Chaoyang, Beijing 100026, China.
Gene. 2019 Jun 5;700:38-46. doi: 10.1016/j.gene.2019.02.089. Epub 2019 Mar 18.
Anti-inflammation is a key process to restore tissue integrity and function. CXCL12 is a homeostasis chemokine, which plays a coordinating role in organogenesis, tumorigenesis and regeneration. In the present study we found that the uterus of abortion mice showed different histo-morphological changes with the development of abortion. The expression of chemokine CXCL12 and its receptor CXCR4 in abortion uterus showed a time-dependent pattern. Compared with normal pregnancy, the expression of CXCL12 and CXCR4 did not change in the uterus of GD7 abortion mice, but increased significantly in the uterus of GD8 and GD10 abortion mice. However, the expression of IFN-γ increased significantly in the uterus of GD7 abortion mice, while there was no significant change detected in GD8 aborted mice uterus. Our further data show that the expression of CXCL12 is not regulated by IFN-γ in endometrial stromal cell culture system in vitro. The treatment of CXCL12 significantly inhibits the expression of IFN-γ in in vitro cultured stromal cells and splenic monocytes. This suggests that CXCL12 may play an anti-inflammatory role in the uterus of abortion mice to promote the process of endometrial restoration after abortion, rather than participate in the process of abortion as a response molecule of IFN-γ.
抗炎是恢复组织完整性和功能的关键过程。趋化因子 CXCL12 是一种内稳态趋化因子，在器官发生、肿瘤发生和再生中发挥协调作用。在本研究中，我们发现流产小鼠的子宫随着流产的发展表现出不同的组织形态学变化。趋化因子 CXCL12 及其受体 CXCR4 在流产子宫中的表达呈时间依赖性模式。与正常妊娠相比，GD7 流产小鼠子宫中 CXCL12 和 CXCR4 的表达没有变化，但在 GD8 和 GD10 流产小鼠子宫中显著增加。然而，IFN-γ 在 GD7 流产小鼠子宫中的表达显著增加，而在 GD8 流产小鼠子宫中未检测到明显变化。我们的进一步数据表明，CXCL12 的表达在体外子宫内膜基质细胞培养系统中不受 IFN-γ 的调节。CXCL12 的处理显著抑制了体外培养的基质细胞和脾单核细胞中 IFN-γ 的表达。这表明 CXCL12 可能在流产小鼠的子宫中发挥抗炎作用，以促进流产后子宫内膜的恢复过程，而不是作为 IFN-γ 的反应分子参与流产过程。
