Department of Applied Chemistry, Faculty of Engineering, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Nanoscale. 2011 Sep 1;3(9):3793-8. doi: 10.1039/c1nr10519a. Epub 2011 Aug 9.
Gold nanorods were fixed on an ITO plate and used for the spectroscopic sensing and Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SALDI-MS) of oligopeptides (angiotensin I). The longitudinal surface plasmon bands of the gold nanorods responded to the 10(-10) M angiotensin solution that was cast on the ITO plate. The SALDI-MS measurements had an ultra-high sensitivity to the angiotensin on the ITO plate. A very small surface density (5 × 10(-19) mol cm(-2)) of angiotensin could be detected at m/z = 1297 with a good signal/noise ratio (S/N = 11). The ITO plate, which was modified with gold nanorods, was found to be effective in collecting angiotensin molecules adjacent to the gold nanorods, and the SALDI processes that were induced by the photoabsorption of the gold nanorods efficiently contributed to the desorption and ionization of the angiotensin.
金纳米棒被固定在 ITO 板上，用于寡肽（血管紧张素 I）的光谱传感和表面辅助激光解吸/离子化飞行时间质谱（SALDI-MS）。金纳米棒的纵向表面等离激元带对涂覆在 ITO 板上的 10（-10）M 血管紧张素溶液有响应。SALDI-MS 测量对 ITO 板上的血管紧张素有超高的灵敏度。在 m/z = 1297 处，可以检测到非常小的表面密度（5×10（-19）mol cm（-2））的血管紧张素，并且具有良好的信噪比（S/N = 11）。ITO 板经金纳米棒修饰后，被发现能够有效地收集与金纳米棒相邻的血管紧张素分子，而金纳米棒的光吸收所诱导的 SALDI 过程有效地促进了血管紧张素的解吸和离子化。
