Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
Langmuir. 2010 Apr 20;26(8):6066-70. doi: 10.1021/la904467b.
A "strategy" for analyte capture/ionization based on chemical derivatization of gold nanorods and infrared laser desorption ionization (IR-LDI) is described. This is the first example of laser desorption/ionization of biomolecules using gold nanorods irradiated with an IR laser. LDI is performed at wavelengths (1064 nm) that overlap with the longitudinal surface plasmon resonance (LSPR) mode of gold nanorods. The absorbed energy from the laser facilitates desorption and ionization of the analyte. The wavelength of the LSPR band can be tuned by controlling the aspect ratio (length-to-diameter) of the nanorod. For example, the SPR band for Au nanorods having an aspect ratio of 5:1 is centered at approximately 840 nm, and this band overlaps with the 1064 nm output of a Nd:YAG laser. We show that a variety of biomolecules can be efficiently desorbed and ionized by 1064 nm irradiation of nanorods. We also show that analyte capture can be controlled by surface chemistry of the nanorods. The results of these studies are important for designing nanomaterial-based capture assays for mass spectrometry and interfacing nanomaterials with imaging/spatial profiling mass spectrometry experiments.
描述了一种基于金纳米棒的化学衍生化和红外激光解吸电离（IR-LDI）的分析物捕获/离解“策略”。这是首次使用 IR 激光辐照金纳米棒进行激光解吸/电离生物分子的示例。LDI 在与金纳米棒的纵向表面等离激元共振（LSPR）模式重叠的波长（1064nm）下进行。激光吸收的能量有助于分析物的解吸和电离。通过控制纳米棒的纵横比（长度与直径之比）可以调节 LSPR 带的波长。例如，纵横比为 5:1 的 Au 纳米棒的 SPR 带的中心位于约 840nm，并且该带与 Nd:YAG 激光的 1064nm 输出重叠。我们表明，通过 1064nm 辐照纳米棒可以有效地解吸和离子化多种生物分子。我们还表明，可以通过纳米棒的表面化学控制分析物的捕获。这些研究的结果对于设计基于纳米材料的质谱分析物捕获测定法以及将纳米材料与成像/空间剖析质谱实验接口非常重要。
