Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Theor Appl Genet. 2011 Dec;123(8):1341-57. doi: 10.1007/s00122-011-1671-z. Epub 2011 Aug 10.
Evolutionary studies in plant and animal breeding are aimed at understanding the structure and organization of genetic variations of species. We have identified and characterized a genomic sequence in Phaseolus vulgaris of 1,200 bp (PvSHP1) that is homologous to SHATTERPROOF-1 (SHP1), a gene involved in control of fruit shattering in Arabidopsis thaliana. The PvSHP1 fragment was mapped to chromosome Pv06 in P. vulgaris and is linked to the flower and seed color gene V. Amplification of the PvSHP1 sequence from the most agronomically important legume species showed a high degree of interspecies diversity in the introns within the Phaseoleae, while the coding region was conserved across distant taxa. Sequencing of the PvSHP1 sequence in a sample of 91 wild and domesticated genotypes that span the geographic distribution of this species in the centers of origin showed that PvSHP1 is highly polymorphic and, therefore, particularly useful to further investigate the origin and domestication history of P. vulgaris. Our data confirm the gene pool structure seen in P. vulgaris along with independent domestication processes in the Andes and Mesoamerica; they provide additional evidence for a single domestication event in Mesoamerica. Moreover, our results support the Mesoamerican origin of this species. Finally, we have developed three indel-spanning markers that will be very useful for bean germplasm characterization, and particularly to trace the distribution of the domesticated Andean and Mesoamerican gene pools.
植物和动物育种的进化研究旨在了解物种遗传变异的结构和组织。我们已经鉴定和描述了菜豆基因组中 1200bp 的一段序列（PvSHP1），该序列与拟南芥果实破碎控制基因 SHATTERPROOF-1（SHP1）同源。PvSHP1 片段被定位在菜豆的 Pv06 染色体上，与花和种子颜色基因 V 连锁。从最具农业重要性的豆科物种中扩增 PvSHP1 序列显示，在 Phaseoleae 内的内含子中存在高度的种间多样性，而编码区在远缘分类群中是保守的。对该物种起源中心地理分布范围内的 91 个野生和栽培基因型的 PvSHP1 序列进行测序表明，PvSHP1 高度多态性，因此特别有助于进一步研究菜豆的起源和驯化历史。我们的数据证实了菜豆中看到的基因库结构，以及安第斯山脉和中美洲的独立驯化过程；它们为中美洲的单一驯化事件提供了额外的证据。此外，我们的结果支持该物种起源于中美洲。最后，我们开发了三个跨越插入缺失的标记，这将非常有助于豆类种质资源的特征描述，特别是追踪驯化的安第斯和中美洲基因库的分布。
