Department of Bionano System Engineering, Chonbuk National University, Jeonju 561-756, South Korea.
J Biomed Nanotechnol. 2011 Jun;7(3):342-50. doi: 10.1166/jbn.2011.1292.
Hydroxyapatite (HA) containing gold (Au) nanoparticles was synthesized for the biomedical applications. The morphology, structure and thermal properties of the HA containing Au nanoparticles were characterized by field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectroscopy, X-ray diffraction (XRD), Fouriertransform infrared spectroscopy (FT-IR) and thermogravimetry (TGA). XRD patterns clearly revealed the formation of HA-Au composite nanoparticles. TEM observation showed that the Au nanoparticles had an average size of 5 nm and were incorporated into HA powder very well. Bactericidal activity of HA-Au with Au nanoparticles immobilized in HA was investigated. The adhesion, viability and proliferation properties of HA containing Au nanoparticles and the differentiation of osteoblast were studied for in vitro cell compatibility of the HA containing Au nanoparticles. Our results showed that HA containing Au nanoparticles were cytotoxic to the human osteoblastic cells.
羟基磷灰石（HA）负载金（Au）纳米颗粒的合成可用于生物医学应用。采用场发射扫描电子显微镜（FE-SEM）、透射电子显微镜（TEM）、能谱（EDX）、X 射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和热重分析（TGA）对负载 Au 纳米颗粒的 HA 的形貌、结构和热性能进行了表征。XRD 图谱清楚地表明了 HA-Au 复合纳米颗粒的形成。TEM 观察表明，Au 纳米颗粒的平均尺寸为 5nm，并且很好地掺入到 HA 粉末中。研究了负载在 HA 中的 Au 纳米颗粒固定化的 HA-Au 的杀菌活性。研究了负载 Au 纳米颗粒的 HA 的黏附、活力和增殖特性，以及成骨细胞的分化，以研究负载 Au 纳米颗粒的 HA 的体外细胞相容性。结果表明，负载 Au 纳米颗粒的 HA 对人成骨细胞具有细胞毒性。
