Research Center of Oils and Fats, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Research Center of Oils and Fats, Kermanshah University of Medical Sciences, Kermanshah, Iran.
J Photochem Photobiol B. 2018 Jul;184:71-79. doi: 10.1016/j.jphotobiol.2018.05.016. Epub 2018 May 19.
Nowadays, green chemistry methods are noticeable for synthesis metal nanoparticles due to affordable, easy and high reaction rate. In the present study gold nanoparticles (Au NPs) were eco-friendly synthesized using Thyme extract at the room temperature for 30 min to provide non-toxic, which can be used for different applications. Identifying properties of synthesized gold nanoparticles was done by various analytical technique including UV-Vis absorption spectroscopy approved presence of Au NPs in the solution, the functional groups of Thyme extract in the reduction and capping process of Au NPs are determined by FT-IR, Crystalline with the fcc plane approved by X-ray diffraction (XRD) pattern, energy dispersive spectroscopy (EDS) determined existence of elements in the sample, surface morphology, diverse shapes and size of present Au NPs were showed by scanning electron microscopy (SEM), atomic force microscopy (AFM) and Transmission electron microscopy (TEM). Beginning and end destroy temperature of the gold nanoparticles were determined by thermal gravimetric spectroscopy (TGA). In addition, antibacterial, antioxidant, and cytotoxicity properties of Au NPs were studied. Antibacterial activity of Au NPs was investigated on gram-positive (Bacillus) and gram-negative (Escherichia coli) by disk diffusion; also MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were determined. DPPH free radical scavenging assay was used for antioxidant property and compared to butylated hydroxy toluene (BHT) as a standard antioxidant that showed high antioxidant activity. Synthesized Au NPs have great cell viability in a dose-depended manner and demonstrate that this method provided nontoxic for synthesis gold nanoparticles. The average diameter of synthesized Au NPs was about 35 nm.
如今，由于成本低、操作简便、反应速率高，绿色化学方法在合成金属纳米粒子方面备受关注。在本研究中，使用百里香提取物在室温下 30 分钟即可环保合成金纳米粒子（Au NPs），提供无毒的金纳米粒子，可用于不同的应用。通过各种分析技术鉴定合成金纳米粒子的特性，包括紫外-可见吸收光谱，证明溶液中存在 Au NPs，傅里叶变换红外光谱（FT-IR）确定了百里香提取物在 Au NPs 的还原和封端过程中的功能团，X 射线衍射（XRD）图案证明了具有 fcc 面的结晶，能谱（EDS）确定了样品中元素的存在，通过扫描电子显微镜（SEM）、原子力显微镜（AFM）和透射电子显微镜（TEM）显示了 Au NPs 的表面形貌、不同形状和尺寸。通过热重分析（TGA）确定了金纳米粒子的起始和结束破坏温度。此外，还研究了 Au NPs 的抗菌、抗氧化和细胞毒性特性。通过圆盘扩散法研究了 Au NPs 对革兰氏阳性（芽孢杆菌）和革兰氏阴性（大肠杆菌）的抗菌活性；还测定了最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。DPPH 自由基清除试验用于抗氧化活性，并与标准抗氧化剂丁基化羟基甲苯（BHT）进行比较，结果表明其具有较高的抗氧化活性。合成的 Au NPs 具有剂量依赖性的高细胞活力，表明该方法提供了无毒的金纳米粒子合成。合成的 Au NPs 的平均直径约为 35nm。
