Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
J Am Chem Soc. 2011 Sep 7;133(35):13914-7. doi: 10.1021/ja2056984. Epub 2011 Aug 16.
The first attempt to prepare biologically active siRNA-based microhydrogels is reported. The self-assembled microhydrogels were fabricated using sense/antisense complementary hybridization between single-stranded linear and Y-shaped trimeric siRNAs. The siRNA microhydrogels were condensed using a popular cationic polymer such as LPEI to form compact, stable siRNA/polymeric nanoparticles that exhibited superb cellular uptake efficiency and gene silencing activity.
首次尝试制备具有生物活性的基于 siRNA 的微凝胶。自组装微凝胶是通过单链线性和 Y 型三聚体 siRNA 之间的互补杂交来制备的。siRNA 微凝胶通过使用流行的阳离子聚合物（如 LPEI）进行缩合，形成紧凑、稳定的 siRNA/聚合物纳米颗粒，表现出优异的细胞摄取效率和基因沉默活性。
