Department of Chemistry, Carleton University, Ottawa, Ontario, Canada.
J Occup Environ Hyg. 2011 Sep;8(9):540-3. doi: 10.1080/15459624.2011.601994.
This study examined the response of various forms and sources of glucans toward two different Limulus amebocyte lysate (LAL) methods, the modified LAL, and Glucatell. The glucans studied were curdlan, laminarin, yeast glucan, barley glucan, paramylon, pullulan, pustulan, mannan, and pachyman (as part of the Glucatell kit). Both methods provided largely similar results for each of the glucans; however, the Glucatell method yielded slightly higher responses to certain structures that may not necessarily be of fungal origin, leading to falsely greater positive results. The performance of each method to measure fungal glucan concentration specifically was then assessed.
本研究考察了不同形式和来源的葡聚糖对两种不同的鲎变形细胞溶解物（LAL）方法，即改良 LAL 和 Glucatell 的反应。所研究的葡聚糖包括几丁质、昆布多糖、酵母葡聚糖、大麦葡聚糖、鱼腥藻葡聚糖、普鲁兰、出芽短梗霉多糖、甘露聚糖和几丁质（作为 Glucatell 试剂盒的一部分）。两种方法对每种葡聚糖的结果基本相似；然而，Glucatell 方法对某些可能并非真菌来源的结构产生的反应略高，导致假阳性结果更高。然后评估了每种方法测量真菌葡聚糖浓度的性能。
