比较嗜酸乳杆菌质粒消除剂的功效。

Comparing the efficacy of plasmid curing agents in Lactobacillus acidophilus.

机构信息

Department of Biotechnology, School of Bioengineering, SRM University, Tamilnadu, India.

出版信息

Benef Microbes. 2010 Jun;1(2):155-8. doi: 10.3920/BM2009.0038.

DOI:10.3920/BM2009.0038

Abstract

This study was mainly focused on plasmid profiling and plasmid characterisation of Lactobacillus acidophilus isolated from molasses. The secondary objective was to observe the efficacy of the plasmid curing agents acriflavine, ethidium bromide, novobiocin and SDS on L. acidophilus. Plasmid-free strains and cured derivatives harbouring only a single plasmid (6.2 kbp) were also obtained. Treatment of L. acidophilus with novobiocin at concentrations of 2.4 µg/ml could produce a large number of chloramphenicol- variants at a very high frequency (4.6%). These curing data confirmed that novobiocin acts as an effective curing agent for L. acidophilus.

摘要

本研究主要集中于从糖蜜中分离的嗜酸乳杆菌的质粒图谱和质粒特征。次要目标是观察吖啶黄素、溴化乙锭、新生霉素和 SDS 等质粒消除剂对嗜酸乳杆菌的功效。还获得了无质粒菌株和仅携带单个质粒（6.2 kbp）的消除衍生菌株。用 2.4 µg/ml 的新生霉素处理嗜酸乳杆菌，可以非常高的频率（4.6%）产生大量氯霉素变体。这些消除数据证实，新生霉素是嗜酸乳杆菌的有效消除剂。