Ruiz-Barba J L, Piard J C, Jiménez-Díaz R
Instituto de la Grasa y sus Derivados (CSIC), UEI de Biotecnología de Alimentos, Sevilla, Spain.
J Appl Bacteriol. 1991 Nov;71(5):417-21. doi: 10.1111/j.1365-2672.1991.tb03810.x.
Plasmid profiles of 35 Lactobacillus plantarum strains isolated from different green olive fermentors were obtained. A large number of plasmids in the CCC form (from 5 to 16) were present in all the tested strains as confirmed by a second dimension electrophoresis of DNA. These plasmids, all of which remain cryptic, ranged from 2.0 to 68 kb in size. Novobiocin, sodium dodecyl sulphate and ethidium bromide were used as plasmid-curing agents but only novobiocin induced loss of extrachromosomal DNA at a high frequency in these strains.
获得了从不同青橄榄发酵罐中分离出的35株植物乳杆菌菌株的质粒图谱。通过DNA二维电泳证实，所有测试菌株中都存在大量CCC形式的质粒（5至16个）。这些质粒均无明显功能，大小在2.0至68 kb之间。使用新生霉素、十二烷基硫酸钠和溴化乙锭作为质粒消除剂，但只有新生霉素能在这些菌株中高频诱导染色体外DNA的丢失。
