Roussel Y, Colmin C, Simonet J M, Decaris B
Laboratoire de Génétique et Microbiologie, Faculté des Sciences, Université Nancy I, Vandoeuvre-lès-Nancy, France.
J Appl Bacteriol. 1993 May;74(5):549-56.
Chromosomal DNA of nine strains of the Lactobacillus acidophilus (Hansen and Mocquot) group in which heterogeneity had previously been revealed by biochemical characteristics and DNA-DNA hybridization studies were further investigated by restriction analysis, Southern hybridization, conventional and pulsed-field gel electrophoresis (PFGE) analyses. Industrial strains were characterized and identified as Lact. acidophilus. For this species, the number of rRNA gene clusters was estimated to be at least four from analyses of rRNA gene restriction patterns. The chromosome size of type-strains of Lact. acidophilus and Lact. gasseri was estimated from PFGE analysis to be 1.85 and 2.02 Mb respectively and Lact. gasseri strains were found to contain large-sized linear plasmids.
先前已通过生化特性和DNA-DNA杂交研究揭示了嗜酸乳杆菌（汉森氏菌和莫科特氏菌）组9个菌株的染色体DNA存在异质性，现通过限制性分析、Southern杂交、常规和脉冲场凝胶电泳（PFGE）分析进一步研究。对工业菌株进行了表征并鉴定为嗜酸乳杆菌。对于该物种，通过rRNA基因限制性图谱分析估计rRNA基因簇的数量至少为四个。嗜酸乳杆菌和加氏乳杆菌模式菌株的染色体大小通过PFGE分析估计分别为1.85 Mb和2.02 Mb，并且发现加氏乳杆菌菌株含有大型线性质粒。
