Falk D R, McCaughin G, Cogley T
Genetics. 1977 Aug;86(4):765-77. doi: 10.1093/genetics/86.4.765.
The genetic and biochemical characteristics of a particular class of mutants at the rudimentary locus are described. The mutants are pyrimidine auxotrophs, like classical rudimentary alleles, but they are unique in that they do not alter the size or shape of the wing (Falk and Nash 1974b). Aspartate transcarbamylase and dihydroorotase activities have been measured in seven different normal-winged mutants, and the results indicate that these strains are enzymologically "leaky" mutants. Previous studies have shown that three genetic functions (corresponding to the first three enzymes of pyrimidine synthesis) are associated with the rudimentary locus. Four of the seven mutants appear to affect all three of these functions. Each of the four is temperature sensitive, and a biochemical analysis of the temperature sensitivity of one of these mutants, (r)pyr1-3, suggests that a process affecting the synthesis or assembly of these enzymes is altered at high temperatures.
本文描述了在粗糙脉孢菌基因座上一类特定突变体的遗传和生化特征。这些突变体是嘧啶营养缺陷型，与经典的粗糙脉孢菌等位基因一样，但它们的独特之处在于不会改变翅膀的大小或形状（福尔克和纳什，1974b）。已对七个不同的正常翅突变体中的天冬氨酸转氨甲酰酶和二氢乳清酸酶活性进行了测定，结果表明这些菌株是酶学上的“渗漏”突变体。先前的研究表明，三种遗传功能（对应于嘧啶合成的前三种酶）与粗糙脉孢菌基因座相关。七个突变体中的四个似乎影响所有这三种功能。这四个突变体中的每一个都是温度敏感型，对其中一个突变体（r）pyr1-3的温度敏感性进行生化分析表明，在高温下，影响这些酶合成或组装的过程发生了改变。
