黑腹果蝇的发育不全位点：氨甲酰磷酸合酶-天冬氨酸转氨甲酰酶-二氢乳清酸酶复合物的部分纯化
Rudimentary locus of Drosophila melanogaster: partial purification of a carbamylphosphate synthase--aspartate transcarbamylase--dihydroorotase complex.
作者信息
Brothers V M, Tsubota S I, Germeraad S E, Fristrom J W
出版信息
Biochem Genet. 1978 Apr;16(3-4):321-32. doi: 10.1007/BF00484088.
Glutamine-dependent CPSase, ATCase, and DHOase from Drosophila, the first three enzymes in pyrimidine biosynthesis, show coordinate variation in activity throughout development. The three activities were highest in first instar larvae and decreased as development proceeded. The three activities cosediment in sucrose gradients as a single peak with a relative sedimentation coefficient of approximately 30S. CPSase, ATCase, and DHOase copurify during (NH4)2SO4 fractionation and during DEAE-cellulose and hydroxylapatite chromatography.
来自果蝇的谷氨酰胺依赖性氨甲酰磷酸合成酶（CPSase）、天冬氨酸转氨甲酰酶（ATCase）和二氢乳清酸酶（DHOase）是嘧啶生物合成中的前三种酶，在整个发育过程中活性呈现协同变化。这三种酶的活性在一龄幼虫中最高，并随着发育进程而降低。这三种酶的活性在蔗糖梯度中共同沉降为一个单一峰，相对沉降系数约为30S。在硫酸铵分级分离以及二乙氨基乙基纤维素（DEAE-纤维素）和羟基磷灰石色谱分离过程中，CPSase、ATCase和DHOase能共同纯化。
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