Frank Duncombe Laboratory, Animal Health Department, IFR146 ICORE, 14053 Caen, France.
J Clin Microbiol. 2011 Oct;49(10):3694-6. doi: 10.1128/JCM.01187-11. Epub 2011 Aug 10.
This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
本研究表明,在核酸提取方法和检测试剂方面具有明确限定条件的情况下,先前描述的实时逆转录聚合酶链反应(rRT-PCR)检测方法(T1 检测方法)在检测精液中的马动脉炎病毒方面的灵敏度与病毒分离法相当或更高。