评价两种基于磁珠的病毒核酸提取试剂盒和三种实时荧光 RT-PCR 试剂系统在两种 TaqMan 检测方法中对精液中马动脉炎病毒检测的效果。
Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen.
机构信息
Frank Duncombe Laboratory, Animal Health Department, IFR146 ICORE, 14053 Caen, France.
出版信息
J Clin Microbiol. 2011 Oct;49(10):3694-6. doi: 10.1128/JCM.01187-11. Epub 2011 Aug 10.
Abstract
This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
摘要
本研究表明，在核酸提取方法和检测试剂方面具有明确限定条件的情况下，先前描述的实时逆转录聚合酶链反应（rRT-PCR）检测方法（T1 检测方法）在检测精液中的马动脉炎病毒方面的灵敏度与病毒分离法相当或更高。
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Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen.评价两种基于磁珠的病毒核酸提取试剂盒和三种实时荧光 RT-PCR 试剂系统在两种 TaqMan 检测方法中对精液中马动脉炎病毒检测的效果。
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