血小板含有肿瘤来源的 RNA 生物标志物。
Blood platelets contain tumor-derived RNA biomarkers.
机构信息
Neuro-oncology Research Group, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.
出版信息
Blood. 2011 Sep 29;118(13):3680-3. doi: 10.1182/blood-2011-03-344408. Epub 2011 Aug 10.
Abstract
Diagnostic platforms providing biomarkers that are highly predictive for diagnosing, monitoring, and stratifying cancer patients are key instruments in the development of personalized medicine. We demonstrate that tumor cells transfer (mutant) RNA into blood platelets in vitro and in vivo, and show that blood platelets isolated from glioma and prostate cancer patients contain the cancer-associated RNA biomarkers EGFRvIII and PCA3, respectively. In addition, gene-expression profiling revealed a distinct RNA signature in platelets from glioma patients compared with normal control subjects. Because platelets are easily accessible and isolated, they may form an attractive platform for the companion diagnostics of cancer.
摘要
诊断平台提供的生物标志物对癌症患者的诊断、监测和分层具有高度预测性，是个性化医疗发展的关键工具。我们证明肿瘤细胞在体外和体内将（突变）RNA转移到血小板中，并表明分离自胶质母细胞瘤和前列腺癌患者的血小板分别含有癌症相关的 RNA 生物标志物 EGFRvIII 和 PCA3。此外，基因表达谱分析显示，与正常对照相比，来自胶质母细胞瘤患者的血小板存在明显不同的 RNA 特征。由于血小板易于获取和分离，因此它们可能成为癌症伴随诊断的有吸引力的平台。
相似文献
1
Blood platelets contain tumor-derived RNA biomarkers.血小板含有肿瘤来源的 RNA 生物标志物。
Blood. 2011 Sep 29;118(13):3680-3. doi: 10.1182/blood-2011-03-344408. Epub 2011 Aug 10.
2
Assessing blood platelets as RNA biomarker source for prostate cancer.评估血小板作为前列腺癌的RNA生物标志物来源。
Biomarkers. 2016 Nov;21(7):653-9. doi: 10.3109/1354750X.2016.1171909. Epub 2016 Apr 28.
3
An integrated transcriptomic and computational analysis for biomarker identification in human glioma.一项用于人类胶质瘤生物标志物识别的综合转录组学和计算分析。
Tumour Biol. 2016 Jun;37(6):7185-92. doi: 10.1007/s13277-015-4585-6. Epub 2015 Dec 14.
4
MXRA5 Is a Novel Immune-Related Biomarker That Predicts Poor Prognosis in Glioma.MXRA5 是一种新型免疫相关生物标志物，可预测胶质瘤预后不良。
Dis Markers. 2021 Jun 9;2021:6680883. doi: 10.1155/2021/6680883. eCollection 2021.
5
FXR1 promotes the malignant biological behavior of glioma cells via stabilizing MIR17HG.FXR1 通过稳定 MIR17HG 促进神经胶质瘤细胞的恶性生物学行为。
J Exp Clin Cancer Res. 2019 Jan 28;38(1):37. doi: 10.1186/s13046-018-0991-0.
6
MicroRNA-30a suppresses self-renewal and tumorigenicity of glioma stem cells by blocking the NT5E-dependent Akt signaling pathway.微小 RNA-30a 通过阻断 NT5E 依赖的 Akt 信号通路抑制神经胶质瘤干细胞的自我更新和致瘤性。
FASEB J. 2020 Apr;34(4):5128-5143. doi: 10.1096/fj.201802629RR. Epub 2020 Feb 17.
7
Glioma-derived extracellular vesicles promote tumor progression by conveying WT1.胶质瘤衍生的细胞外囊泡通过传递 WT1 促进肿瘤进展。
Carcinogenesis. 2020 Sep 24;41(9):1238-1245. doi: 10.1093/carcin/bgaa052.
8
MiRNA expression profiling in human gliomas: upregulated miR-363 increases cell survival and proliferation.人类胶质瘤中的微小RNA表达谱分析：上调的miR-363可提高细胞存活率和增殖能力。
Tumour Biol. 2016 Oct;37(10):14035-14048. doi: 10.1007/s13277-016-5273-x. Epub 2016 Aug 6.
9
Transforming growth factor beta induced (TGFBI) is a potential signature gene for mesenchymal subtype high-grade glioma.转化生长因子β诱导（TGFBI）是间充质亚型高级别神经胶质瘤的一个潜在特征基因。
J Neurooncol. 2018 Apr;137(2):395-407. doi: 10.1007/s11060-017-2729-9. Epub 2018 Jan 2.
10
Let-7a suppresses glioma cell proliferation and invasion through TGF-β/Smad3 signaling pathway by targeting HMGA2.Let-7a通过靶向HMGA2，经由TGF-β/Smad3信号通路抑制胶质瘤细胞的增殖和侵袭。
Tumour Biol. 2016 Jun;37(6):8107-19. doi: 10.1007/s13277-015-4674-6. Epub 2015 Dec 29.
引用本文的文献
1
Tumor-educated platelet RNA as a diagnostic biomarker for ground-glass opacity-related lung adenocarcinoma.肿瘤诱导血小板RNA作为磨玻璃影相关肺腺癌的诊断生物标志物
Transl Lung Cancer Res. 2025 Aug 31;14(8):3090-3106. doi: 10.21037/tlcr-2025-747. Epub 2025 Aug 26.
2
Navigating the Landscape of Liquid Biopsy in Colorectal Cancer: Current Insights and Future Directions.探索结直肠癌液体活检领域：当前见解与未来方向
Int J Mol Sci. 2025 Aug 6;26(15):7619. doi: 10.3390/ijms26157619.
3
Molecular Mechanisms of Tumor Progression and New Therapeutic Strategies for Urological Cancers, 2nd Edition.《肿瘤进展的分子机制与泌尿系统癌症的新治疗策略》第二版
Int J Mol Sci. 2025 Jul 5;26(13):6496. doi: 10.3390/ijms26136496.
4
Role of Liquid Biopsy for Early Detection, Prognosis, and Therapeutic Monitoring of Hepatocellular Carcinoma.液体活检在肝细胞癌早期检测、预后评估及治疗监测中的作用
Diagnostics (Basel). 2025 Jun 28;15(13):1655. doi: 10.3390/diagnostics15131655.
5
A promising frontier of circulating messenger RNA in liquid biopsy: From mechanisms to clinical applications.液体活检中循环信使核糖核酸的一个前沿领域：从机制到临床应用
Int J Cancer. 2025 Oct 15;157(8):1519-1537. doi: 10.1002/ijc.35523. Epub 2025 Jun 19.
6
Promises and pitfalls of multi-cancer early detection using liquid biopsy tests.使用液体活检检测进行多癌早期检测的前景与陷阱
Nat Rev Clin Oncol. 2025 Jun 13. doi: 10.1038/s41571-025-01033-x.
7
Platelets and inflammation-insights from platelet non-coding RNA content and release in the Bruneck study and the PACMAN-AMI trial.血小板与炎症——来自布伦内克研究及心肌梗死血小板图谱研究（PACMAN-AMI）中血小板非编码RNA含量及释放情况的见解
Cardiovasc Res. 2025 Aug 14;121(9):1392-1406. doi: 10.1093/cvr/cvaf100.
8
Liquid biopsy in gynecological cancers: a translational framework from molecular insights to precision oncology and clinical practice.妇科癌症中的液体活检：从分子洞察到精准肿瘤学及临床实践的转化框架
J Exp Clin Cancer Res. 2025 May 8;44(1):140. doi: 10.1186/s13046-025-03371-1.
9
Tumor-Educated Platelets in Urological Tumors: A Novel Biosource in Liquid Biopsy.泌尿系统肿瘤中的肿瘤衍生血小板：液体活检中的新型生物来源
Int J Mol Sci. 2025 Apr 11;26(8):3595. doi: 10.3390/ijms26083595.
10
Liquid biopsies for the monitoring of gliomas and brain metastases in adults.用于监测成人胶质瘤和脑转移瘤的液体活检
Acta Neuropathol. 2025 Apr 26;149(1):37. doi: 10.1007/s00401-025-02880-9.
本文引用的文献
1
Shift in the balance between circulating thrombospondin-1 and vascular endothelial growth factor in cancer patients: Relationship to platelet a-granule content and primary activation.癌症患者循环血中血小板反应蛋白-1与血管内皮生长因子平衡的改变：与血小板α颗粒含量及初始激活的关系
Int J Biol Markers. 2004 Jul-Sep;19(3):221-228. doi: 10.5301/JBM.2008.1959.
2
Megakaryocytes differentially sort mRNAs for matrix metalloproteinases and their inhibitors into platelets: a mechanism for regulating synthetic events.巨核细胞将基质金属蛋白酶及其抑制剂的 mRNA 有差异地分拣到血小板中：一种调节合成事件的机制。
Blood. 2011 Aug 18;118(7):1903-11. doi: 10.1182/blood-2010-12-324517. Epub 2011 May 31.
3
Significant downregulation of platelet gene expression in metastatic lung cancer.转移性肺癌中血小板基因表达显著下调。
Clin Transl Sci. 2010 Oct;3(5):227-32. doi: 10.1111/j.1752-8062.2010.00226.x.
4
MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins.微小 RNA 经血浆运输，并通过高密度脂蛋白递送至受体细胞。
Nat Cell Biol. 2011 Apr;13(4):423-33. doi: 10.1038/ncb2210. Epub 2011 Mar 20.
5
Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity.血小板 microRNA-mRNA 共表达谱与血小板反应性相关。
Blood. 2011 May 12;117(19):5189-97. doi: 10.1182/blood-2010-09-299719. Epub 2011 Mar 17.
6
Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma.Argonaute2 复合物在人类血浆中不依赖于囊泡携带一组循环 microRNAs。
Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):5003-8. doi: 10.1073/pnas.1019055108. Epub 2011 Mar 7.
7
Contribution of platelets to tumour metastasis.血小板在肿瘤转移中的作用。
Nat Rev Cancer. 2011 Feb;11(2):123-34. doi: 10.1038/nrc3004.
8
A novel role for platelet secretion in angiogenesis: mediating bone marrow-derived cell mobilization and homing.血小板分泌在血管生成中的新作用：介导骨髓来源细胞的动员和归巢。
Blood. 2011 Apr 7;117(14):3893-902. doi: 10.1182/blood-2010-08-304808. Epub 2011 Jan 11.
9
Exosomes: Fit to deliver small RNA.外泌体：适合递送小RNA。
Commun Integr Biol. 2010 Sep;3(5):447-50. doi: 10.4161/cib.3.5.12339.
10
Microparticles in cancer.癌症中的微粒
Semin Thromb Hemost. 2010 Nov;36(8):888-906. doi: 10.1055/s-0030-1267043. Epub 2010 Nov 3.
Zotero 插件