Gangadharan Rajan, Anandan Venkataramani, Zhang Guigen
Micro/Nano Bioengineering Lab, Department of Biological and Agricultural Engineering, The University of Georgia, Athens, GA 30602, USA.
Nanotechnology. 2008 Oct 1;19(39):395501. doi: 10.1088/0957-4484/19/39/395501. Epub 2008 Aug 11.
In this study, the functionalization process for nanopillar enhanced electrodes (NEEs) using glucose oxidase (GOx) with polypyrrole (PPY) is optimized for the purpose of achieving enhanced sensing performances for these electrodes in glucose detection. Specifically, an optimal roughness factor for the NEEs and an optimal set of electro-polymerization/deposition parameters for their functionalization using GOx/PPY are identified. Results show that NEEs with a roughness factor of about 60 are optimal for enhancing the amperometric current responses and that for such electrodes an electro-functionalization/deposition process at a deposition current of 50 µA cm(-2) and a total charge of 150 mC cm(-2) will give rise to a high sensing performance with a sensitivity as high as 36 µA cm(-2) mM(-1).
在本研究中，为了提高纳米柱增强电极（NEEs）在葡萄糖检测中的传感性能，对使用葡萄糖氧化酶（GOx）和聚吡咯（PPY）对NEEs进行功能化的过程进行了优化。具体而言，确定了NEEs的最佳粗糙度因子以及使用GOx/PPY对其进行功能化的最佳电聚合/沉积参数集。结果表明，粗糙度因子约为60的NEEs对于增强安培电流响应是最佳的，对于此类电极，在沉积电流为50 μA cm⁻²和总电荷量为150 mC cm⁻²的情况下进行电功能化/沉积过程，将产生高达36 μA cm⁻² mM⁻¹的灵敏度的高传感性能。
