Choi Inhee, Kim Younghun, Kim Jong Ho, Yang Young In, Lee Jeongjin, Lee Suseung, Hong Surin, Yi Jongheop
School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 151-742, Korea.
Nanotechnology. 2008 Nov 5;19(44):445701. doi: 10.1088/0957-4484/19/44/445701. Epub 2008 Sep 30.
High speed imaging by atomic force microscopy (AFM) allows one to directly observe the dynamic behavior of a sample surface immersed in liquid media; thus, it has been considered to be an indispensable tool for nanobiotechnology and is used in many research fields, including molecular biology and surface science. For real-time observation of a certain behavior, the high speed imaging technique should be accompanied with a high resolution imaging technique to identify target materials. To improve the image quality at a high scanning rate, we developed a variable-controlled fast scanning method, which originated from the modified squeeze-drag superposition model in liquid media. A collection of non-distorted images was accomplished after proper modification of the operating conditions in a viscous fluid, via the simple handling of loading force and cantilever length. Consequently, a speeded-up AFM imaging process was achieved in the liquid environment at up to 200 µm s(-1), without attachment of additional devices. The reliability of the proposed method was verified by the characterization of a grating sample immersed in three types of liquid media. In addition, the results were visualized for elastic biomolecules submerged in a liquid with high kinematic viscosity.
通过原子力显微镜（AFM）进行的高速成像能够让人直接观察浸没在液体介质中的样品表面的动态行为；因此，它被认为是纳米生物技术中不可或缺的工具，并被应用于包括分子生物学和表面科学在内的许多研究领域。为了实时观察特定行为，高速成像技术应辅以高分辨率成像技术以识别目标材料。为了在高扫描速率下提高图像质量，我们开发了一种可变控制的快速扫描方法，该方法源自液体介质中改进的挤压 - 拖曳叠加模型。通过简单地处理加载力和悬臂长度，在粘性流体中对操作条件进行适当修改后，获得了一组无失真的图像。因此，在不附加额外设备的情况下，在液体环境中实现了高达200 µm s⁻¹的加速AFM成像过程。通过对浸没在三种液体介质中的光栅样品进行表征，验证了所提出方法的可靠性。此外，还对浸没在具有高运动粘度的液体中的弹性生物分子的结果进行了可视化。
