Division of Immunodermatology and Allergy Research, Department of Dermatology and Allergy, Hannover Medical School, Hannover, Germany.
Int Arch Allergy Immunol. 2011;156(4):412-5. doi: 10.1159/000323905. Epub 2011 Aug 10.
Patients with atopic dermatitis (AD) are frequently colonized with α-toxin-producing Staphylococcus aureus which is in turn positively correlated with the severity of eczema.
IN this study we addressed T cell proliferation and T cell as well as monocyte cytokine secretion upon α-toxin stimulation in peripheral blood mononuclear cells (PBMCs) from AD patients compared to healthy controls.
We found that α-toxin stimulation of PBMCs markedly enhanced T cell proliferation both in patients with AD and healthy controls and was significantly increased in AD patients compared to healthy controls. PBMCs of AD patients secreted significantly more IL-31 compared to those of healthy controls upon α-toxin and SEB stimulation. Moreover, α-toxin stimulation yielded an increase in T cell (IL-2, IL-9, IL-10 and IFN-γ) as well as monocyte (IL-1β and TNF-α) cytokine secretion.
Our results could partly explain how skin colonization and infection with S. aureus can contribute to chronic skin inflammation and pruritus in AD.
特应性皮炎（AD）患者常定植有产α-毒素的金黄色葡萄球菌，而金黄色葡萄球菌的定植与湿疹的严重程度呈正相关。
在这项研究中，我们研究了与健康对照组相比，AD 患者外周血单个核细胞（PBMC）在α-毒素刺激下的 T 细胞增殖以及 T 细胞和单核细胞细胞因子的分泌情况。
我们发现，α-毒素刺激 PBMC 均可明显增强 AD 患者和健康对照组的 T 细胞增殖，且 AD 患者的增强程度明显高于健康对照组。与健康对照组相比，α-毒素和 SEB 刺激后，AD 患者的 PBMC 分泌的 IL-31 明显更多。此外，α-毒素刺激可增加 T 细胞（IL-2、IL-9、IL-10 和 IFN-γ）和单核细胞（IL-1β 和 TNF-α）细胞因子的分泌。
我们的结果部分解释了金黄色葡萄球菌定植和感染如何导致 AD 慢性皮肤炎症和瘙痒。
