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如何将微量移液器集成到封闭的微流体系统中：光学捕获红细胞的吸收光谱。

How to integrate a micropipette into a closed microfluidic system: absorption spectra of an optically trapped erythrocyte.

作者信息

Alrifaiy Ahmed, Ramser Kerstin

出版信息

Biomed Opt Express. 2011 Aug 1;2(8):2299-306. doi: 10.1364/BOE.2.002299. Epub 2011 Jul 20.

DOI:10.1364/BOE.2.002299
PMID:21833366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3149527/
Abstract

We present a new concept of integrating a micropipette within a closed microfluidic system equipped with optical tweezers and a UV-Vis spectrometer. A single red blood cell (RBC) was optically trapped and steered in three dimensions towards a micropipette that was integrated in the microfluidic system. Different oxygenation states of the RBC, triggered by altering the oxygen content in the microchannels through a pump system, were optically monitored by a UV-Vis spectrometer. The built setup is aimed to act as a multifunctional system where the biochemical content and the electrophysiological reaction of a single cell can be monitored simultaneously. The system can be used for other applications like single cell sorting, in vitro fertilization or electrophysiological experiments with precise environmental control of the gas-, and chemical content.

摘要

我们提出了一种将微量移液器集成到配备光镊和紫外可见光谱仪的封闭微流控系统中的新概念。单个红细胞（RBC）被光镊捕获并在三维空间中导向集成在微流控系统中的微量移液器。通过泵系统改变微通道中的氧气含量来触发红细胞的不同氧合状态，并用紫外可见光谱仪进行光学监测。所构建的装置旨在作为一个多功能系统，可同时监测单个细胞的生化成分和电生理反应。该系统还可用于其他应用，如单细胞分选、体外受精或在精确控制气体和化学成分的环境下进行电生理实验。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/3fcb793763fd/boe-2-8-2299-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/017214a12eb4/boe-2-8-2299-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/a613b2d17cda/boe-2-8-2299-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/57c5f2abba9f/boe-2-8-2299-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/31d5434570ee/boe-2-8-2299-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/3fcb793763fd/boe-2-8-2299-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/017214a12eb4/boe-2-8-2299-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/a613b2d17cda/boe-2-8-2299-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/57c5f2abba9f/boe-2-8-2299-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/31d5434570ee/boe-2-8-2299-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c93b/3149527/3fcb793763fd/boe-2-8-2299-g005.jpg

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