Dpto. Química Física Aplicada, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain.
Biol Trace Elem Res. 2011 Dec;144(1-3):1302-17. doi: 10.1007/s12011-011-9140-8. Epub 2011 Jul 22.
A semiquantitative ICP-MS method suitable for evaluating metal content in plants exposed to high metal concentrations is described. The methodology which has been tested using different plant reference material is able, in only a few minutes, to obtain qualitative and quantitative information from the sample. Recoveries close to 100% were obtained for more than half of the referenced elements with an RSD <10%, thus avoiding the generation of a calibration line for each of the elements to be analyzed. The use of multi-standards improves the results obtained with conventional standards by a factor of two. In these conditions the semiquantitative multi-elemental analysis is a valid alternative for most of the analyzed elements with the exception of Si, Al, and P which requires the use of a quantitative ICP-MS analysis for its determination. Two different digestion protocols were tested, with and without the addition of HF to the HNO(3) + H(2)O(2) extraction mixture. Similar results were obtained for poplar and bush reference materials using the HNO(3) + H(2)O(2) mixture. The addition of HF did not improve the recovery of different elements, in general, but is critical for the quantitative measurements of Al and Si as its presence allows results to be obtained with an RDS <5%.
描述了一种适用于评估暴露于高浓度金属环境中的植物金属含量的半定量 ICP-MS 方法。该方法已经使用不同的植物参考材料进行了测试，仅需几分钟即可从样品中获得定性和定量信息。对于超过一半的参考元素，回收率接近 100%，相对标准偏差（RSD）<10%，从而避免了为每个要分析的元素生成校准线。使用多标准可以将常规标准的结果提高两倍。在这些条件下，半定量多元素分析是大多数分析元素的有效替代方法，除了 Si、Al 和 P 之外，它们需要使用定量 ICP-MS 分析来确定。测试了两种不同的消解方案，一种是在 HNO3+H2O2 提取混合物中加入 HF，另一种是不加 HF。使用 HNO3+H2O2 混合物对杨树和灌木参考材料进行了类似的研究，结果相似。HF 的添加通常不会提高不同元素的回收率，但对于 Al 和 Si 的定量测量至关重要，因为 HF 的存在可以使结果的 RDS<5%。
