Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
Calcif Tissue Int. 2011 Oct;89(4):271-84. doi: 10.1007/s00223-011-9516-y. Epub 2011 Jul 22.
The key to treating steroid-induced necrosis of femoral heads (SINFH) is early diagnosis. Dramatic improvements in diagnosis could be made if the pathogenesis of SINFH was more fully understood; however, the underlying mechanism of this disease is currently unknown. To explore the potential mechanism of SINFH, we performed gene array analysis on a rat model of the disease and compare the expression profile with that of normal rats. A quantitative RT-PCR and immunohistochemistry (IHC) assays were used to confirm the microarray results. Compared to the control group, 190 genes in the experimental group were differentially expressed, with 52 up-regulated and 138 down-regulated. Of these genes, 102 are known (deposited in GenBank), while 88 of them are unknown. The known genes can be divided into several families according to their biological functions, such as oxidative stress, apoptosis, signal transduction, angiogenesis, extracellular matrix, lipid metabolism, and transcription related genes. The results of quantitative RT-PCR and IHC were consistent with gene chip results. Our findings indicate that many genes involved in diverse signaling pathways were differentially expressed between SINFH rats and normal rats. Furthermore, our findings suggest that the development of SINFH is a complicated and dynamic process affected by multiple factors and signaling pathways and regulated by various genes.
治疗激素性股骨头坏死（SINFH）的关键是早期诊断。如果能更充分地了解 SINFH 的发病机制，诊断水平将会显著提高；然而，目前尚不清楚这种疾病的潜在机制。为了探讨 SINFH 的潜在机制，我们对激素性股骨头坏死大鼠模型进行了基因芯片分析，并将其表达谱与正常大鼠进行了比较。采用实时定量 RT-PCR 和免疫组织化学（IHC）检测对芯片结果进行了验证。与对照组相比，实验组有 190 个基因差异表达，其中 52 个上调，138 个下调。这些基因中，已知的（已存入 GenBank）有 102 个，未知的有 88 个。已知基因可根据其生物学功能分为几个家族，如氧化应激、细胞凋亡、信号转导、血管生成、细胞外基质、脂质代谢和转录相关基因。实时定量 RT-PCR 和 IHC 的结果与基因芯片结果一致。我们的研究结果表明，SINFH 大鼠和正常大鼠之间存在许多参与不同信号通路的差异表达基因。此外，我们的研究结果表明，SINFH 的发展是一个受多种因素和信号通路影响的复杂和动态过程，受多种基因调控。
