Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, P.R. China.
Department of Orthopedic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Mol Med Rep. 2017 Dec;16(6):9331-9336. doi: 10.3892/mmr.2017.7808. Epub 2017 Oct 17.
The mechanism of steroid-associated femoral head necrosis remains unclear. The present study investigated the role of microRNA-23a-3p (miR-23a-3p) in the incidence of osteonecrosis in a rat model. An miR-23a-3p mimic, an inhibitor and a negative control were transfected into bone mesenchymal stem cells using a lentiviral vector, and then injected into the steroid-induced femoral head necrosis model. Osteonecrosis incidence was assessed by micro computed tomography and histopathology. Low-density lipoprotein receptor-related protein 5 (LRP-5) expression was assessed by immunohistochemistry. The results demonstrated the incidence of osteonecrosis decreased in the miR-23a-3p inhibitor group compared with the miR-23a-3p mimic group (18.2% vs. 75%; P<0.05). The ratio of bone volume/total volume and trabecular thickness were significantly increased in the miR-23a-3p inhibitor group compared with the miR-23a mimic group. The expression level of LRP-5 was higher in the miR-23a-3p inhibitor group. The present study indicated that miR may provide a novel and alternative approach for understanding the mechanism underlying steroid-associated necrosis of the femoral head.
类固醇相关股骨头坏死的发病机制尚不清楚。本研究探讨了 microRNA-23a-3p（miR-23a-3p）在大鼠模型股骨头坏死发病中的作用。通过慢病毒载体转染 miR-23a-3p 模拟物、抑制剂和阴性对照物到骨髓间充质干细胞中，然后将其注入类固醇诱导的股骨头坏死模型中。通过 microCT 和组织病理学评估骨坏死的发生率。通过免疫组化评估低密度脂蛋白受体相关蛋白 5（LRP-5）的表达。结果表明，与 miR-23a-3p 模拟物组相比，miR-23a-3p 抑制剂组的骨坏死发生率降低（18.2%比 75%；P<0.05）。miR-23a-3p 抑制剂组的骨体积/总体积比和小梁厚度比明显高于 miR-23a 模拟物组。miR-23a-3p 抑制剂组 LRP-5 的表达水平较高。本研究表明，miR 可能为了解类固醇相关股骨头坏死的发病机制提供了一种新的、可供选择的方法。
