Debeer Serena
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Methods Mol Biol. 2011;766:165-76. doi: 10.1007/978-1-61779-194-9_11.
X-ray absorption spectroscopy (XAS) involves the excitation of core electrons to bound states localized on the photoabsorber and the eventual excitation of the photoelectron to the continuum. The resulting spectra are typically divided into two regions: (1) the edge region which provides electronic structure information and (2) the extended X-ray absorption fine structure (EXAFS) region, which provides information about the distance, number, and type of ligands. Here, a basic introduction to XAS theory, the information that can be obtained, and the experimental consideration are presented. The application of XAS to biological systems and the impact this has had on nitrogenase research are briefly highlighted. New experimental advances are described.
X射线吸收光谱法（XAS）涉及将内层电子激发到光吸收体上的束缚态，以及最终将光电子激发到连续区。所得光谱通常分为两个区域：（1）提供电子结构信息的边缘区域，以及（2）提供有关配体的距离、数量和类型信息的扩展X射线吸收精细结构（EXAFS）区域。本文介绍了XAS理论的基本内容、可获得的信息以及实验方面的考虑因素。简要强调了XAS在生物系统中的应用及其对固氮酶研究的影响。还描述了新的实验进展。
