Institute for Composite and Biomedical Materials (IMCB), CNR, P.le Tecchio 80, 80125 Naples, Italy.
J Agric Food Chem. 2011 Sep 28;59(18):10062-70. doi: 10.1021/jf201728p. Epub 2011 Aug 24.
The effect of alkaline lignin (AL) and sodium lignosulfonate (LSS) on the structure of thermoplastic zein (TPZ) was studied. Protein structural changes and the nature of the physical interaction between lignin and zein were investigated by means of X-ray diffraction and Fourier transform infrared (FT-IR) spectroscopy and correlated with physical properties. Most relevant protein structural changes were observed at low AL concentration, where strong H-bondings between the functional groups of AL and the amino acids in zein induced a destructuring of inter- and intramolecular interactions in α-helix, β-sheet, and β-turn secondary structures. This destructuring allowed for an extensive protein conformational modification which, in turn, resulted in a strong improvement of the physical properties of the bionanocomposite.
研究了堿木质素（AL）和木质素磺酸钠（LSS）对热塑性玉米醇溶蛋白（TPZ）结构的影响。通过 X 射线衍射和傅里叶变换红外（FT-IR）光谱研究了木质素与玉米醇溶蛋白之间的物理相互作用和蛋白质结构变化，并与物理性能相关联。在低 AL 浓度下观察到最相关的蛋白质结构变化，AL 中的官能团与玉米醇溶蛋白中的氨基酸之间的强氢键导致α-螺旋、β-折叠和β-转角二级结构中分子间和分子内相互作用的破坏。这种破坏允许蛋白质构象的广泛修饰，进而导致生物纳米复合材料物理性能的显著提高。
