Genome Sequencing and Analysis Program, Broad Institute, Cambridge, MA 02142, USA.
Genome Biol. 2011 Aug 11;12(8):R73. doi: 10.1186/gb-2011-12-8-r73.
We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximately 40-fold enrichment of parasite DNA after hybrid selection. This approach will enable efficient genome sequencing of pathogens from clinical samples, as well as sequencing of endosymbiotic organisms such as Wolbachia that live inside diverse metazoan phyla.
我们已经改编了一种溶液杂交选择方案，以富集以人类遗传物质为主的临床样本中的病原体 DNA。使用人类和恶性疟原虫疟原虫 DNA 的模拟混合物以及来自感染患者的临床样本，我们证明杂交选择后寄生虫 DNA 的平均富集约为 40 倍。这种方法将能够从临床样本中有效测序病原体，以及对生活在各种后生动物门内的共生生物（如沃尔巴克氏体）进行测序。
