Garin-Chesa P, Beresford H R, Walker S, Rettig W J
Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Am J Pathol. 1990 Apr;136(4):797-807.
The A4 and AO10 (110 kd glycoprotein) cell-surface antigens are biochemically distinct markers of cultured human astrocytomas that are expressed by only a limited number of other cultured cell types. To further characterize these two antigens, the authors used immunohistochemical methods to determine their expression in normal human tissues, astrocytomas, and over 100 tumors of other histologic types. They found that A4 is expressed 1) throughout the central (CNS), but not peripheral nervous system (PNS); 2) in smooth muscle and a small number of epithelial tissues; and 3) in reactive glia and in astrocytomas, but not in most tumors of other histologic types. In contrast, the AO10 antigen is expressed 1) in a small subset of CNS neurons, but not in astrocytes, PNS neurons, or other normal tissues; 2) in astrocytomas and reactive glia; and 3) in some additional neuroectodermal tumors, but not melanomas, carcinomas, or sarcomas. These findings show that A4 and AO10 are restricted markers for human astrocytomas in vivo. Furthermore, the antigens show distinct patterns of expression in normal human CNS but appear to be coordinately expressed in astrocytomas and astrocytoma-derived cell lines.
A4和AO10（110kd糖蛋白）细胞表面抗原是培养的人类星形细胞瘤的生化特性不同的标志物，仅在有限数量的其他培养细胞类型中表达。为了进一步表征这两种抗原，作者使用免疫组织化学方法来确定它们在正常人体组织、星形细胞瘤以及100多种其他组织学类型肿瘤中的表达情况。他们发现，A4在以下情况中表达：1）遍布中枢神经系统（CNS），但不在周围神经系统（PNS）中表达；2）在平滑肌和少数上皮组织中表达；3）在反应性胶质细胞和星形细胞瘤中表达，但在大多数其他组织学类型的肿瘤中不表达。相比之下，AO10抗原在以下情况中表达：1）在一小部分中枢神经系统神经元中表达，但不在星形胶质细胞、周围神经系统神经元或其他正常组织中表达；2）在星形细胞瘤和反应性胶质细胞中表达；3）在一些其他神经外胚层肿瘤中表达，但不在黑色素瘤、癌或肉瘤中表达。这些发现表明，A4和AO10在体内是人类星形细胞瘤的限制性标志物。此外，这些抗原在正常人类中枢神经系统中表现出不同的表达模式，但在星形细胞瘤和星形细胞瘤衍生的细胞系中似乎是协同表达的。
