雌激素与改变的饮食硬度对大鼠髁突软骨中雌激素受体α和基质金属蛋白酶-8表达的影响

Effect of estrogen and altered diet hardness on the expression of estrogen receptor alpha and matrix metalloproteinase-8 in rat condylar cartilage.

作者信息

Orajarvi Marko, Hirvonen Outi, Yu Shi-Bin, Liu Xiadong, Tiilikainen Petri, Wang Meiqing, Raustia Aune, Pirttiniemi Pertti

机构信息

Department of Prosthetic Dentistry and Stomatognathic Physiology, School of Stomatology, Fourth Military Medical Univeristy, Xi'an China.

出版信息

J Orofac Pain. 2011 Summer;25(3):261-8.

PMID:21837293

Abstract

AIMS

To examine the effect of decreased estrogen level and altered diet hardness on condylar cartilage morphology of the rat temporomandibular joint (TMJ) and on the expression of condylar cartilage estrogen receptor alpha (ERa) and matrix metalloproteinase-8 (MMP-8).

METHODS

A total of 36 female rats was divided into four groups: ovariectomized rats fed a normal diet, non-ovariectomized controls fed a normal diet, ovariectomized rats fed a soft diet, and non-ovariectomized controls fed a soft diet. Ovariectomy was performed at the age of 60 days. Seven days after the operation, the rats were sacrificed. Repeated measures ANOVA and Duncan's multiple comparison tests were used for statistical analysis.

RESULTS

The ovariectomized rats had thicker cartilage layers than the controls, both in the normal diet and soft diet groups. The thinnest cartilage layers were found in the control rats fed with the soft diet. The thickness of the chondroblastic layer was significantly higher (P < .001) in the normal-diet rats than in the soft-diet rats in both ovariectomized and non-ovariectomized groups. The thickness of the proliferative layer was significantly higher (P < .001) in the ovariectomized soft-diet rats than in the soft-diet control rats. The proportional amount of ERa was statistically significantly higher (P < .001) in the condylar cartilage of the ovariectomized rats than in the non-ovariectomized control rats both in the normal- and soft-diet groups. The proportional amount of ERa was statistically significantly higher (P < .001) in the ovariectomized normal-diet rats than in the ovariectomized soft-diet rats. The proportional number of MMP-8-positive cells was statistically significantly higher (P < .001) in the condylar cartilage of ovariectomized rats fed the soft diet than in non-ovariectomized control rats fed the soft diet. Control rats fed with the normal diet had a higher proportional amount of MMP-8 positive cells than control rats fed with the soft diet (P < .05).

CONCLUSION

The rat TMJ condylar cartilage is sensitive to changes in estrogen levels and altered diet hardness.

摘要

目的

研究雌激素水平降低和饮食硬度改变对大鼠颞下颌关节（TMJ）髁突软骨形态以及髁突软骨雌激素受体α（ERα）和基质金属蛋白酶-8（MMP-8）表达的影响。

方法

将36只雌性大鼠分为四组：卵巢切除后喂正常饮食的大鼠、未卵巢切除的喂正常饮食的对照组、卵巢切除后喂软食的大鼠、未卵巢切除的喂软食的对照组。60日龄时进行卵巢切除术。术后7天处死大鼠。采用重复测量方差分析和邓肯多重比较检验进行统计学分析。

结果

在正常饮食组和软食组中，卵巢切除的大鼠软骨层均比对照组厚。喂食软食的对照大鼠软骨层最薄。在卵巢切除组和未卵巢切除组中，正常饮食大鼠的成软骨细胞层厚度均显著高于软食大鼠（P <.001）。卵巢切除的软食大鼠增殖层厚度显著高于软食对照大鼠（P <.001）。在正常饮食组和软食组中，卵巢切除大鼠髁突软骨中ERα的比例均显著高于未卵巢切除的对照大鼠（P <.001）。卵巢切除的正常饮食大鼠中ERα的比例显著高于卵巢切除的软食大鼠（P <.001）。喂食软食的卵巢切除大鼠髁突软骨中MMP-8阳性细胞的比例显著高于喂食软食的未卵巢切除对照大鼠（P <.001）。喂食正常饮食的对照大鼠MMP-8阳性细胞的比例高于喂食软食的对照大鼠（P <.05）。