Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Antalya Bilim University, Antalya, Turkey.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Karadeniz Technical University, Trabzon, Turkey.
BMC Oral Health. 2024 Oct 8;24(1):1193. doi: 10.1186/s12903-024-04990-4.
Oxidative damage and inflammatory cytokines in osteoarthritis (OA) exacerbate the disease course. Daidzein (DZ) has antioxidant and anti-inflammatory effects. This study evaluated the early histopathological effects of intra-articular daidzein injection on experimentally induced osteoarthritis in rabbit TMJs.
The predictor variable was intra-articular injection of DZ or a saline control. 50 µl of 3 mg/mL MIA solution was injected into the right TMJ of 16 New Zealand rabbits to induce experimental OA. One rabbit was sacrificed after 4 weeks to confirm the formation of the OA model and the OA model was obtained. The remaining 15 rabbits were randomly divided into 2 groups: an experimental group (9 rabbits) and a control group (6 rabbits). On days 1, 7, 14, and 21; 50 µl of saline solution was applied to the right TMJ of the control group and 50 µl daidzein solution (1.8 mg/ml) was applied to the right TMJ to the experimental group. After one week from the date of the last injection, the rabbits were sacrificed, and histopathological and biochemical evaluations were performed. The Shapiro-Wilk test was used to evaluate whether the variables in the study conformed to normal distribution. Mean ± SD (standard deviation) or median (interquartile range (IQR)) was used to show the descriptive statistics of the variables. T-test and Mann Whitney U test were used to compare the control and experimental groups for biochemical changes. The chi-square test was used to show the distribution of histopathological changes variables obtained within the scope of the study based on control and experimental groups. A P-value < 0.05 was considered significant for all evaluations.
There were 8 and 6 animate treated with DZ and saline, respectively. There was no statistically significant difference between groups in articular cartilage (p = 0.3), osteochondral junction (p = 0.3), subchondral bone structure (p = 1.0) or chondrocyte appearance (p = 0.4). The experimental group showed significantly lower mean values for Total Oxidant Status (TOS) (p = 0.002) and Oxidative Stress Index (OSI) (p = 0.007).
An intra-articular DZ injection appears to show limited reduction of oxidative damage and early OA in the rabbit TMJ. DZ might represent a promising natural compound with beneficial effects in the management of TMJ-OA.
骨关节炎（OA）中的氧化损伤和炎性细胞因子会加重疾病进程。大豆苷元（DZ）具有抗氧化和抗炎作用。本研究评估了关节内注射大豆苷元对兔颞下颌关节（TMJ）实验性 OA 的早期组织病理学影响。
预测变量为关节内注射 DZ 或生理盐水对照。将 50µl 3mg/ml MIA 溶液注入 16 只新西兰兔的右侧 TMJ 中，以诱导实验性 OA。4 周后处死 1 只兔子以确认 OA 模型的形成，并获得 OA 模型。其余 15 只兔子随机分为 2 组：实验组（9 只兔子）和对照组（6 只兔子）。在第 1、7、14 和 21 天；对照组右侧 TMJ 给予 50µl 生理盐水，实验组右侧 TMJ 给予 50µl 大豆苷元溶液（1.8mg/ml）。末次注射后 1 周，处死兔子，进行组织病理学和生化评估。Shapiro-Wilk 检验用于评估研究中的变量是否符合正态分布。平均值±标准差（SD）或中位数（四分位距（IQR））用于显示变量的描述性统计。T 检验和 Mann-Whitney U 检验用于比较对照组和实验组的生化变化。卡方检验用于显示基于对照组和实验组的研究范围内获得的组织病理学变化变量的分布。所有评估的 P 值均<0.05 被认为具有统计学意义。
分别有 8 只和 6 只兔子用 DZ 和生理盐水处理。关节软骨（p=0.3）、骨软骨交界处（p=0.3）、软骨下骨结构（p=1.0）或软骨细胞外观（p=0.4）在两组之间无统计学差异。实验组总氧化状态（TOS）（p=0.002）和氧化应激指数（OSI）（p=0.007）的平均值明显较低。
关节内注射 DZ 似乎可显示出对兔 TMJ 中氧化损伤和早期 OA 的有限减轻。DZ 可能是一种有前途的天然化合物，对 TMJ-OA 的治疗具有有益作用。
