Jones Alexandra M E, Nühse Thomas S
The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
Methods Mol Biol. 2011;779:287-302. doi: 10.1007/978-1-61779-264-9_17.
The identification of phosphorylation on proteins has become practicable for many laboratories in recent years, largely due to improvements in mass spectrometry (MS) and the development of methods to selectively enrich for phosphorylated peptides and proteins. However, phosphorylation is a dynamic and reversible modification which plays a central role in many biological processes including intracellular signalling. Therefore, the quantitative analysis of phosphorylated proteins and peptides is a subject of intense interest. We discuss three applications of isobaric tags for relative and absolute quantitation (iTRAQ) to the analysis of phosphopeptides from a variety of sample materials.
近年来，蛋白质磷酸化的鉴定对许多实验室来说已变得切实可行，这主要归功于质谱（MS）技术的改进以及选择性富集磷酸化肽段和蛋白质方法的发展。然而，磷酸化是一种动态且可逆的修饰，在包括细胞内信号传导在内的许多生物过程中起着核心作用。因此，磷酸化蛋白质和肽段的定量分析是一个备受关注的课题。我们讨论了用于相对和绝对定量的等压标记（iTRAQ）在分析来自各种样品材料的磷酸肽方面的三种应用。
