Zhang Shuncang, Liu Yan, Shen Shuang, Liang Zongsuo, Yang Dongfeng
College of Life Sciences, Northwest Agriculture & Forestry University, Yangling 712100, China.
Zhongguo Zhong Yao Za Zhi. 2011 May;36(10):1269-74.
To observe the effects of a biotic elicitor fungal hyphae extract, an abiotic elicitor methyl jasmonate and their synergistic action on the accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza hairy root.
Different elicitors were added to S. miltiorrhiza hairy root, which was subcultured for 21 days, the dry weight and contents of phenolic acids and tanshinones were determined at different harvest-time.
S. miltiorrhiza hairy root growth was significantly inhibited by all three treatments and the accumulation of cryptotanshinone and dihydrotanshinone were promoted by each elicition. As for the accumulation of phenolic acids, there were differences between fungal elicitor and methyl jasmonate treatments, they were promoted by methyl jasmonate while inhibited in a certain extent by fungal hyphae extract.
Fungal elicitor, methyl jasmonate and their synergistic action have significant influence on accumulation of components in S. miltiorrhiza hairy root, and the effect varies between phenolic acids and tanshinones. There is no correlation between production of water-soluble ingredients and fat-soluble components on the whole under three different treatments.
观察生物诱导子真菌菌丝提取物、非生物诱导子茉莉酸甲酯及其协同作用对丹参毛状根中酚酸和丹参酮积累的影响。
将不同诱导子添加到丹参毛状根中，继代培养21天，在不同收获时间测定其干重以及酚酸和丹参酮的含量。
三种处理均显著抑制丹参毛状根生长，每种诱导处理均促进隐丹参酮和二氢丹参酮的积累。对于酚酸的积累，真菌诱导子和茉莉酸甲酯处理存在差异，茉莉酸甲酯促进其积累，而真菌菌丝提取物在一定程度上抑制其积累。
真菌诱导子、茉莉酸甲酯及其协同作用对丹参毛状根中成分的积累有显著影响，且对酚酸和丹参酮的影响有所不同。三种不同处理下，水溶性成分和脂溶性成分的产生总体上没有相关性。
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