Wang Cong Hui, Zheng Li Ping, Tian Hao, Wang Jian Wen
College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China.
Department of Horticulture, Soochow University, Suzhou 215123, China.
J Photochem Photobiol B. 2016 Jun;159:93-100. doi: 10.1016/j.jphotobiol.2016.01.012. Epub 2016 Mar 17.
Tanshinones are major bioactive diterpenoids of Salvia miltiorrhiza roots used for the treatment of cardiocerebral diseases. To develop effective elicitation and bioprocess strategies for the enhanced production of tanshinones, ultraviolet-B (UV-B) irradiation and methyl jasmonate (MeJA) elicitation were applied alone or in combination respectively in S. miltiorrhiza hairy root cultures. Our results showed 40-min UV-B irradiation at 40μW/cm(2) stimulated tanshinone production without any suppression of root growth, suggesting a new effective elicitor to S. miltiorrhiza hairy root cultures for tanshinone production. Moreover, the combined treatment of UV-B irradiation and MeJA exhibited synergistic effects on the expression levels of 3-hydroxy-3-methylglutaryl-CoA reductase (SmHMGR) and geranylgeranyl diphosphate synthase (SmGGPPS) genes in the tanshinone biosynthetic pathway. When hairy roots of 18-day-old cultures were exposed to the combined elicitation for 9days, the maximum production of tanshinone reached to 28.21mg/L, a 4.9-fold increase over the control. The combined elicitation of UV-B and MeJA was firstly used to stimulate the production of biologically important secondary metabolites in hairy root cultures.
丹参酮是丹参根中的主要生物活性二萜类化合物，用于治疗心脑血管疾病。为了开发有效的诱导和生物工艺策略以提高丹参酮的产量，分别单独或联合应用紫外线-B（UV-B）照射和茉莉酸甲酯（MeJA）诱导处理丹参毛状根培养物。我们的结果表明，40μW/cm(2)的UV-B照射40分钟可刺激丹参酮的产生，而不会对根生长产生任何抑制，这表明UV-B是丹参毛状根培养物中用于丹参酮生产的一种新的有效诱导剂。此外，UV-B照射和MeJA的联合处理对丹参酮生物合成途径中3-羟基-3-甲基戊二酰辅酶A还原酶（SmHMGR）和香叶基香叶基二磷酸合酶（SmGGPPS）基因的表达水平具有协同作用。当18天龄培养物的毛状根接受联合诱导9天时，丹参酮的最大产量达到28.21mg/L，比对照增加了4.9倍。UV-B和MeJA的联合诱导首次用于刺激毛状根培养物中具有生物学重要性的次生代谢产物的产生。
