茉莉酸甲酯对丹参毛状根悬浮培养物中丹参酮积累与释放的影响
[Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root].
作者信息
Wang Xue-Yong, Cui Guang-Hong, Huang Lu-Qi, Qiu De-You
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(4):300-2.
OBJECTIVE
To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots.
METHOD
After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC.
RESULT
After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0.039, 0.204, 0.571 mg x g(-1) respectively,and tanshinone II(A) reached 0.251, 0.601 and 1.563 mg x g(-1) respectively. After 9 day's treatment by MJ, the maximum increase of cryptotanshinone and tanshinone II(A) were 23.8 fold and 6.2 fold higher than that of the control respectively.
CONCLUSION
MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.
目的
研究茉莉酸甲酯（MJ）对丹参毛状根悬浮培养物中丹参酮积累和释放的影响。
方法
用发根农杆菌ATCC15834诱导丹参毛状根进行18天的悬浮培养后，向6 - 7V悬浮培养物中添加化学诱导剂——茉莉酸甲酯，同时，采用高效液相色谱法对处理MJ后第2、6和9天的丹参酮含量（包括隐丹参酮和丹参酮II（A））进行定量分析。
结果
在第2、6和9天处理MJ后，隐丹参酮浓度分别达到0.039、0.204、0.571 mg·g⁻¹，丹参酮II（A）分别达到0.251、0.601和1.563 mg·g⁻¹。经MJ处理9天后，隐丹参酮和丹参酮II（A）的最大增加量分别比对照高23.8倍和6.2倍。
结论
MJ能刺激丹参中丹参酮的积累并将其释放到培养基中。
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