Chen Xing, Yang Guangming, Zhang Fangfang, Wang Mingyan, He Yuanyuan, Cai Baochang
Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2011 May;36(10):1362-5.
To examine apoptosis of SMMC-7721 hepatocarcinoma cells induced by total flavonoids of Oxytropis falcata (TFOF) and its preliminary mechanism.
SMMC-7721 cells were treated for 24 h with TFOF in different concentrations. Inhibition on proliferation of SMMC-7721 cells was assessed by MTT assay. The morphology of treated SMMC-7721 cells was observed by optical microscope. Effect of TFOF on the nuclear morphology of cells was analyzed using Hoechst 33258 staining by fluorescence microscope. Annexin V-FITC/PI staining and flow cytometric measurement were used for investigating the effect of TFOF on induction of apoptosis in SMMC-7721 cells and cell cycle analysis.
The results of MTT assay showed that TFOF could induce cytotoxicity in SMMC-7721 cells in a dose-dependent manner. Hoechst 33258 staining analysis indicated that TFOF caused typical characteristics of apoptotic programmed cell death, such as cell shrinkage, apoptotic body formation etc. Flow cytometric analysis demonstrated that TFOF caused a dose-dependent apoptosis of SMMC-7721 cells and arrested cell cycle in G1 phase.
It suggested that TFOF inhibit proliferation of SMMC-7721 cells by inducing apoptosis of the cells and arresting cell cycle in G1 phase.
研究镰形棘豆总黄酮（TFOF）诱导人肝癌SMMC - 7721细胞凋亡及其初步机制。
用不同浓度的TFOF处理SMMC - 7721细胞24小时。采用MTT法检测TFOF对SMMC - 7721细胞增殖的抑制作用。用光学显微镜观察经处理的SMMC - 7721细胞的形态。采用Hoechst 33258染色，通过荧光显微镜分析TFOF对细胞细胞核形态的影响。采用Annexin V - FITC/PI染色和流式细胞术检测TFOF对SMMC - 7721细胞凋亡的诱导作用及细胞周期分析。
MTT法结果显示，TFOF可呈剂量依赖性地诱导SMMC - 7721细胞产生细胞毒性。Hoechst 33258染色分析表明，TFOF引起典型的凋亡程序性细胞死亡特征，如细胞皱缩、凋亡小体形成等。流式细胞术分析表明，TFOF可呈剂量依赖性地诱导SMMC - 7721细胞凋亡，并使细胞周期阻滞于G1期。
提示TFOF可能通过诱导SMMC - 7721细胞凋亡及使细胞周期阻滞于G1期来抑制其增殖。
