[中国红豆杉培养细胞提取物的抗肿瘤活性研究]
[Study on anti-tumor activity of extracts from cultured cells of Taxus chinensis].
作者信息
Lu Cuitao, Mei Xingguo
机构信息
School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074.
出版信息
Zhong Yao Cai. 2003 May;26(5):335-7.
OBJECTIVE
To analyze the cytotoxicity and inhibitory effect of extracts from cultured cells(from stems) of Taxus chinensis on cancer cell line SMMC-7721.
METHODS
MTT assay for cell viability and flow cytometry for cell cycle analysis.
RESULTS
MTT results showed that the extact by dichloromethane was more effective than that by methanol on SMMC-7721, IC50 were 0.0834 mg DCW.ml-1 and 0.8002 mg DCW.ml-1 respectively. SMMC-7721 cells in G2-M stage increased with higher concentration of dichloromethane extracts from Taxus chinensis cells and longer incubation.
CONCLUSION
Extracts from cultured cells of Taxus chinensis could have cytotoxic effect on SMMC-7721 and could induce apoptosis of cancer cells.
目的
分析中国红豆杉（茎）培养细胞提取物对癌细胞系SMMC - 7721的细胞毒性和抑制作用。
方法
采用MTT法检测细胞活力，流式细胞术分析细胞周期。
结果
MTT结果显示，二氯甲烷提取物对SMMC - 7721的作用比甲醇提取物更有效，IC50分别为0.0834 mg DCW·ml-1和0.8002 mg DCW·ml-1。随着中国红豆杉细胞二氯甲烷提取物浓度的升高和孵育时间的延长，处于G2 - M期的SMMC - 7721细胞增多。
结论
中国红豆杉培养细胞提取物对SMMC - 7721具有细胞毒性作用，并可诱导癌细胞凋亡。
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