Allergy and Immunology Section, Institute of Genomics and Integrative Biology (CSIR), Delhi, India.
J Agric Food Chem. 2011 Sep 28;59(18):9990-5. doi: 10.1021/jf202265d. Epub 2011 Aug 31.
Genetic engineering can enhance abiotic stress tolerance of plants, thereby increasing productivity. The present study investigates allergenicity of osmotin protein used for developing transgenic crops. Bioinformatic analysis of osmotin was performed using SDAP and Farrp allergen databases. Osmotin was cloned in pET22b+ vector, purified to homogeneity, and analyzed for digestibility, heat stability, and IgE binding using atopic patients' sera. Osmotin showed 40-92% and 48-75% homology with allergens in SDAP and Farrp databases, respectively. These cross-reactive allergens were from apple, tomato, peach, capsicum, kiwi fruit, and cypress. Osmotin was resistant to pepsin digestion and heat treatment at 90 °C for 1 h. Osmotin protein showed dose-dependent inhibition with pooled patients' sera. It showed significant IgE binding with 22 of 117 patients' sera who were sensitized to tomato and apple, thus indicating cross-reactivity among tomato, apple, and osmotin allergens. In conclusion, osmotin was identified as a potential allergen and showed cross-reactivity with tomato and apple allergens.
基因工程可以提高植物的非生物胁迫耐受性，从而提高生产力。本研究调查了用于开发转基因作物的渗透蛋白的变应原性。使用 SDAP 和 Farrp 过敏原数据库对渗透蛋白进行了生物信息学分析。渗透蛋白被克隆到 pET22b+载体中，进行了纯化以达到均一性，并使用过敏患者的血清分析了其消化率、热稳定性和 IgE 结合能力。渗透蛋白与 SDAP 和 Farrp 数据库中的过敏原分别具有 40-92%和 48-75%的同源性。这些交叉反应性过敏原来自苹果、番茄、桃、辣椒、猕猴桃和柏树。渗透蛋白能抵抗胃蛋白酶消化和 90°C 热处理 1 小时。渗透蛋白与 117 份对番茄和苹果过敏的患者血清中的 22 份血清呈剂量依赖性抑制作用，表明番茄、苹果和渗透蛋白过敏原之间存在交叉反应性。总之，渗透蛋白被鉴定为一种潜在的过敏原，与番茄和苹果过敏原具有交叉反应性。
