James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
Cancer Lett. 2011 Dec 1;311(1):66-76. doi: 10.1016/j.canlet.2011.06.033. Epub 2011 Jul 13.
Pituitary tumor-transforming gene (PTTG) is an oncogene with its expression levels correlating with tumor development and metastasis. Epithelial to mesenchymal transition (EMT) is a crucial step in tumor progression and metastasis. Using ovarian epithelial tumor cell line (A2780) for loss-of-function or gain-of-function of PTTG in our experiments, we observed up regulation of TGF-β, Twist, Snail, Slug, vimentin and down regulation of E-cadherin on infection of cells with Ad-PTTG cDNA. In contrast reverse phenomena was observed on depletion of PTTG on infection of cells with Ad-PTTG siRNA, suggesting an important role of PTTG in induction of EMT in ovarian cancer cells.
垂体肿瘤转化基因（PTTG）是一种癌基因，其表达水平与肿瘤的发生和转移相关。上皮间质转化（EMT）是肿瘤进展和转移的关键步骤。在我们的实验中，使用卵巢上皮肿瘤细胞系（A2780）对 PTTG 进行功能丧失或功能获得，我们观察到感染 Ad-PTTG cDNA 的细胞中 TGF-β、Twist、Snail、Slug、波形蛋白上调，E-钙黏蛋白下调。相反，在用 Ad-PTTG siRNA 感染细胞时，PTTG 的缺失观察到相反的现象，表明 PTTG 在诱导卵巢癌细胞 EMT 中起重要作用。
