改良的非侵入性微测试电生理技术用于检测液泡 H(+)流。

Modified noninvasive microtest electrophysiological technology for vacuolar H(+) flux detection.

机构信息

Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China.

出版信息

Anal Biochem. 2011 Nov 15;418(2):295-7. doi: 10.1016/j.ab.2011.07.018. Epub 2011 Jul 22.

DOI:10.1016/j.ab.2011.07.018

PMID:21839717

Abstract

This paper describes a modified noninvasive microtest electrophysiological technology (NMT) for vacuolar H(+) flux detection. In this NMT system, the vacuole isolation procedure and buffer slope were modified, and the measuring errors from small spherical geometry were corrected. The trends in changes of vacuolar H(+) flux (ΔH(+) flux) after ATP or PP(i) supply calculated by NMT were consistent with the activities of V-ATPase and PPase measured by traditional methods. These findings indicate that our modified NMT is an appropriate method for vacuolar H(+) flux detection.

摘要

本文描述了一种改良的非侵入性微测试电生理学技术（NMT），用于检测液泡 H(+)流。在这个 NMT 系统中，我们修改了液泡分离程序和缓冲斜率，并校正了小球形几何测量误差。通过 NMT 计算得出的 ATP 或 PP(i)供应后液泡 H(+)流变化趋势（ΔH(+) flux）与传统方法测量的 V-ATPase 和 PPase 活性一致。这些发现表明，我们改良的 NMT 是一种检测液泡 H(+)流的合适方法。