• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蚊子中表达的咽侧体激素受体的功能特征。

Functional characterization of an allatotropin receptor expressed in the corpora allata of mosquitoes.

机构信息

Dept. Biological Sciences, Florida International University, Miami, FL 33199, USA.

出版信息

Peptides. 2012 Mar;34(1):201-8. doi: 10.1016/j.peptides.2011.07.025. Epub 2011 Aug 3.

DOI:10.1016/j.peptides.2011.07.025
PMID:21839791
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3233642/
Abstract

Allatotropin is an insect neuropeptide with pleiotropic actions on a variety of different tissues. In the present work we describe the identification, cloning and functional and molecular characterization of an Aedes aegypti allatotropin receptor (AeATr) and provide a detailed quantitative study of the expression of the AeATr gene in the adult mosquito. Analysis of the tissue distribution of AeATr mRNA in adult female revealed high transcript levels in the nervous system (brain, abdominal, thoracic and ventral ganglia), corpora allata-corpora cardiaca complex and ovary. The receptor is also expressed in heart, hindgut and male testis and accessory glands. Separation of the corpora allata (CA) and corpora cardiaca followed by analysis of gene expression in the isolated glands revealed expression of the AeATr primarily in the CA. In the female CA, the AeATr mRNA levels were low in the early pupae, started increasing 6h before adult eclosion and reached a maximum 24h after female emergence. Blood feeding resulted in a decrease in transcript levels. The pattern of changes of AeATr mRNA resembles the changes in JH biosynthesis. Fluorometric Imaging Plate Reader recordings of calcium transients in HEK293 cells expressing the AeATr showed a selective response to A. aegypti allatotropin stimulation in the low nanomolar concentration range. Our studies suggest that the AeATr play a role in the regulation of JH synthesis in mosquitoes.

摘要

阿莱托菌素是一种昆虫神经肽，对多种不同组织具有多种作用。在本工作中，我们描述了埃及伊蚊阿莱托菌素受体（AeATr）的鉴定、克隆以及功能和分子特征，并对成蚊中 AeATr 基因的表达进行了详细的定量研究。分析成年雌性蚊子中 AeATr mRNA 的组织分布，发现其在神经系统（脑、腹部、胸部和腹神经节）、咽侧体-心侧体复合体和卵巢中具有高转录水平。该受体还在心脏、后肠和雄性睾丸和附腺中表达。分离咽侧体（CA）和心侧体后，分析分离的腺体中的基因表达，发现 AeATr 主要在 CA 中表达。在雌性 CA 中，AeATr mRNA 水平在早期蛹中较低，在成虫羽化前 6 小时开始增加，并在雌性出现后 24 小时达到最大值。吸血导致转录水平下降。AeATr mRNA 的变化模式与 JH 生物合成的变化相似。在表达 AeATr 的 HEK293 细胞中进行钙瞬变荧光成像板读数记录显示，对 A. aegypti 阿莱托菌素的刺激在低纳摩尔浓度范围内具有选择性反应。我们的研究表明，AeATr 在蚊子中 JH 合成的调节中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/c97d2b6a82d3/nihms320967f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/549da3ca8eac/nihms320967f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/468967b8d4ff/nihms320967f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/5b51c8abf424/nihms320967f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/dd3fe0bddf6c/nihms320967f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/c22112ecfd3e/nihms320967f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/c97d2b6a82d3/nihms320967f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/549da3ca8eac/nihms320967f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/468967b8d4ff/nihms320967f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/5b51c8abf424/nihms320967f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/dd3fe0bddf6c/nihms320967f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/c22112ecfd3e/nihms320967f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f3/3233642/c97d2b6a82d3/nihms320967f6.jpg

相似文献

1
Functional characterization of an allatotropin receptor expressed in the corpora allata of mosquitoes.蚊子中表达的咽侧体激素受体的功能特征。
Peptides. 2012 Mar;34(1):201-8. doi: 10.1016/j.peptides.2011.07.025. Epub 2011 Aug 3.
2
Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedes allatotropin.成年雌性埃及伊蚊咽侧体体外刺激保幼激素生物合成：法尼酸和埃及伊蚊促咽侧体素的作用
J Exp Biol. 2003 Jun;206(Pt 11):1825-32. doi: 10.1242/jeb.00371.
3
Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes.在蚊虫咽侧体中表达的保幼激素酸甲基转移酶的分子和功能特征
Insect Biochem Mol Biol. 2009 Jan;39(1):31-7. doi: 10.1016/j.ibmb.2008.09.010. Epub 2008 Oct 17.
4
Allatostatin-C receptors in mosquitoes.蚊子中的阿塔斯丁-C 受体。
Peptides. 2010 Mar;31(3):442-50. doi: 10.1016/j.peptides.2009.04.013. Epub 2009 May 3.
5
Allatotropin inhibits juvenile hormone biosynthesis by the corpora allata of adult Bombyx mori.促咽侧体素抑制成年家蚕咽侧体的保幼激素生物合成。
J Insect Physiol. 2015 Sep;80:15-21. doi: 10.1016/j.jinsphys.2015.01.005. Epub 2015 Jan 21.
6
A coordinated expression of biosynthetic enzymes controls the flux of juvenile hormone precursors in the corpora allata of mosquitoes.生物合成酶的协调表达控制了蚊子咽侧体中保幼激素前体的通量。
Insect Biochem Mol Biol. 2011 Sep;41(9):660-9. doi: 10.1016/j.ibmb.2011.04.008. Epub 2011 May 3.
7
The pleiotropic allatoregulatory neuropeptides and their receptors: A mini-review.多效性咽侧体调节神经肽及其受体：一篇综述短文
J Insect Physiol. 2015 Sep;80:2-14. doi: 10.1016/j.jinsphys.2015.04.004. Epub 2015 May 15.
8
Identification and functional characterization of FGLamide-related allatostatin receptor in Rhodnius prolixus.罗得西亚锥蝽中FGL酰胺相关咽侧体抑制素受体的鉴定及功能特性研究
Insect Biochem Mol Biol. 2015 Feb;57:1-10. doi: 10.1016/j.ibmb.2014.12.001. Epub 2014 Dec 11.
9
Ecdysis triggering hormone ensures proper timing of juvenile hormone biosynthesis in pharate adult mosquitoes.蜕皮触发激素确保即将羽化的成年蚊子中保幼激素生物合成的时间恰当。
Insect Biochem Mol Biol. 2014 Nov;54:98-105. doi: 10.1016/j.ibmb.2014.09.006. Epub 2014 Sep 23.
10
Characterisation of a functional allatotropin receptor in the bumblebee, Bombus terrestris (Hymenoptera, Apidae).在熊蜂（膜翅目，蜜蜂科）中鉴定一个功能性的促前胸腺激素受体。
Gen Comp Endocrinol. 2013 Nov 1;193:193-200. doi: 10.1016/j.ygcen.2013.08.006. Epub 2013 Aug 19.

引用本文的文献

1
G-Protein Coupled Receptors (GPCRs) in Insects-A Potential Target for New Insecticide Development.昆虫 G 蛋白偶联受体（GPCRs）-新型杀虫剂开发的潜在靶标。
Molecules. 2021 May 18;26(10):2993. doi: 10.3390/molecules26102993.
2
G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology.G蛋白偶联受体（GPCRs）：昆虫生理学和毒理学中的信号通路、特性及功能
Int J Mol Sci. 2021 May 17;22(10):5260. doi: 10.3390/ijms22105260.
3
Glycoprotein Hormone Receptor Knockdown Leads to Reduced Reproductive Success in Male .

本文引用的文献

1
Characterization of an allatotropin-like peptide receptor in the red flour beetle, Tribolium castaneum.直译为：在赤拟谷盗（Tribolium castaneum）中鉴定一种促前胸腺激素类似肽受体。 意译为：鉴定赤拟谷盗中一种促前胸腺激素类似肽受体。
Insect Biochem Mol Biol. 2011 Oct;41(10):815-22. doi: 10.1016/j.ibmb.2011.06.003. Epub 2011 Jul 1.
2
Isolation and functional characterization of an allatotropin receptor from Manduca sexta.从烟草天蛾中分离和功能表征一种脑激素受体。
Insect Biochem Mol Biol. 2011 Oct;41(10):804-14. doi: 10.1016/j.ibmb.2011.06.002. Epub 2011 Jun 14.
3
A coordinated expression of biosynthetic enzymes controls the flux of juvenile hormone precursors in the corpora allata of mosquitoes.
糖蛋白激素受体敲低导致雄性生殖成功率降低。
Front Physiol. 2019 Mar 19;10:266. doi: 10.3389/fphys.2019.00266. eCollection 2019.
4
Transcriptomic analysis of the prothoracic gland from two lepidopteran insects, domesticated silkmoth Bombyx mori and wild silkmoth Antheraea pernyi.转录组分析两种鳞翅目昆虫，驯化家蚕 Bombyx mori 和野生野蚕 Antheraea pernyi 的前胸腺。
Sci Rep. 2019 Mar 29;9(1):5313. doi: 10.1038/s41598-019-41864-0.
5
Mosquito Peptide Hormones: Diversity, Production, and Function.蚊子肽类激素：多样性、产生与功能
Adv In Insect Phys. 2016;51:145-188. doi: 10.1016/bs.aiip.2016.05.003. Epub 2016 Jun 14.
6
Omics approaches to study juvenile hormone synthesis.研究保幼激素合成的组学方法。
Curr Opin Insect Sci. 2018 Oct;29:49-55. doi: 10.1016/j.cois.2018.05.013. Epub 2018 May 26.
7
Discovery of a Manduca sexta Allatotropin Antagonist from a Manduca sexta Allatotropin Receptor Homology Model.从烟夜蛾前胸腺激素受体同源模型中发现烟夜蛾前胸腺激素拮抗剂。
Molecules. 2018 Apr 3;23(4):817. doi: 10.3390/molecules23040817.
8
Allatotropin: A pleiotropic neuropeptide that elicits mosquito immune responses.保幼激素促泌素：一种引发蚊子免疫反应的多效性神经肽。
PLoS One. 2017 Apr 20;12(4):e0175759. doi: 10.1371/journal.pone.0175759. eCollection 2017.
9
The current state of knowledge on the neuroactive compounds that affect the development, mating and reproduction of spiders (Araneae) compared to insects.与昆虫相比，关于影响蜘蛛（蜘蛛目）发育、交配和繁殖的神经活性化合物的当前知识状态。
Invert Neurosci. 2017 Jun;17(2):4. doi: 10.1007/s10158-017-0197-8. Epub 2017 Apr 18.
10
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae.转录组测序揭示无菌埃及伊蚊幼虫的大规模变化。
PLoS Negl Trop Dis. 2017 Jan 6;11(1):e0005273. doi: 10.1371/journal.pntd.0005273. eCollection 2017 Jan.
生物合成酶的协调表达控制了蚊子咽侧体中保幼激素前体的通量。
Insect Biochem Mol Biol. 2011 Sep;41(9):660-9. doi: 10.1016/j.ibmb.2011.04.008. Epub 2011 May 3.
4
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.MEGA5：用于最大似然法、进化距离法和最大简约法的分子进化遗传学分析。
Mol Biol Evol. 2011 Oct;28(10):2731-9. doi: 10.1093/molbev/msr121. Epub 2011 May 4.
5
Orexin A stimulates glucose uptake, lipid accumulation and adiponectin secretion from 3T3-L1 adipocytes and isolated primary rat adipocytes.食欲素 A 可刺激 3T3-L1 脂肪细胞和分离的原代大鼠脂肪细胞的葡萄糖摄取、脂质积累和脂联素分泌。
Diabetologia. 2011 Jul;54(7):1841-52. doi: 10.1007/s00125-011-2152-2. Epub 2011 Apr 21.
6
The molecular receptive ranges of human TAS2R bitter taste receptors.人类 TAS2R 苦味受体的分子接受范围。
Chem Senses. 2010 Feb;35(2):157-70. doi: 10.1093/chemse/bjp092. Epub 2009 Dec 18.
7
The human bitter taste receptor hTAS2R50 is activated by the two natural bitter terpenoids andrographolide and amarogentin.人类苦味受体 hTAS2R50 可被两种天然苦味萜烯类化合物穿心莲内酯和当药苦苷激活。
J Agric Food Chem. 2009 Nov 11;57(21):9860-6. doi: 10.1021/jf9014334.
8
Control of the release of digestive enzymes in the larvae of the fall armyworm, Spodoptera frugiperda.控制秋粘虫幼虫中消化酶的释放。
Arch Insect Biochem Physiol. 2010 Jan;73(1):14-29. doi: 10.1002/arch.20332.
9
Neuropeptide regulators of juvenile hormone synthesis: structures, functions, distribution, and unanswered questions.保幼激素合成的神经肽调节因子：结构、功能、分布及未解决的问题
Ann N Y Acad Sci. 2009 Apr;1163:316-29. doi: 10.1111/j.1749-6632.2009.04459.x.
10
Spatial expression of the mevalonate enzymes involved in juvenile hormone biosynthesis in the corpora allata in Bombyx mori.家蚕咽侧体中参与保幼激素生物合成的甲羟戊酸途径酶的空间表达。
J Insect Physiol. 2009 Sep;55(9):798-804. doi: 10.1016/j.jinsphys.2009.04.013. Epub 2009 May 18.