Reynolds P, Koken M H, Hoeijmakers J H, Prakash S, Prakash L
Department of Biophysics, University of Rochester School of Medicine, NY 14642.
EMBO J. 1990 May;9(5):1423-30. doi: 10.1002/j.1460-2075.1990.tb08258.x.
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enzyme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxyl-terminal 21 amino acids present in the RAD6 protein are absent in the rhp6(+)-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.
酿酒酵母的RAD6基因编码一种泛素结合酶，在DNA修复、DNA损伤诱导的诱变和孢子形成过程中发挥作用。在此，我们发现，RAD6与远缘酵母粟酒裂殖酵母中的rhp6 +基因在结构和功能上具有高度同源性。RAD6蛋白中主要为酸性的羧基末端21个氨基酸在rhp6(+)编码的蛋白中不存在；除此之外，这两种蛋白非常相似，有77%的相同残基。与rad6一样，rhp6 +基因的无效突变会导致DNA修复、紫外线诱变和孢子形成缺陷，并且RAD6和rhp6 +基因在功能上可以相互替代。这些观察结果表明，RAD6（rhp6 +）蛋白与DNA修复复合体其他成分之间的功能相互作用在真核生物中是保守的。
