Koken M, Reynolds P, Bootsma D, Hoeijmakers J, Prakash S, Prakash L
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Proc Natl Acad Sci U S A. 1991 May 1;88(9):3832-6. doi: 10.1073/pnas.88.9.3832.
The RAD6 gene of the yeast Saccharomyces cerevisiae is required for DNA repair, for DNA damage-induced mutagenesis, and for sporulation, and it encodes a ubiquitin-conjugating enzyme. We have cloned the RAD6 homolog from Drosophila melanogaster and find that its encoded protein displays a very high degree of identity in amino acid sequence with the homologous RAD6 proteins from the two divergent yeasts, S. cerevisiae and Schizosaccharomyces pombe, and from human. Genetic complementation studies indicate that the Drosophila RAD6 homolog can functionally substitute for the S. cerevisiae RAD6 gene in its DNA-repair and UV-mutagenesis functions but cannot substitute in sporulation. The high degree of structural and functional conservation of RAD6 in eukaryotic evolution suggests that the various protein components involved in RAD6-dependent DNA repair and mutagenesis functions have also been conserved.
酿酒酵母的RAD6基因对于DNA修复、DNA损伤诱导的诱变以及孢子形成是必需的，并且它编码一种泛素结合酶。我们已经从黑腹果蝇中克隆出RAD6同源物，发现其编码的蛋白质在氨基酸序列上与来自两种不同酵母（酿酒酵母和粟酒裂殖酵母）以及人类的同源RAD6蛋白质具有非常高的同一性。遗传互补研究表明，果蝇RAD6同源物在其DNA修复和紫外线诱变功能上可以在功能上替代酿酒酵母的RAD6基因，但在孢子形成方面不能替代。RAD6在真核生物进化中的高度结构和功能保守性表明，参与RAD6依赖性DNA修复和诱变功能的各种蛋白质成分也得到了保守。
