School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
J Pharm Biomed Anal. 2011 Dec 5;56(4):785-91. doi: 10.1016/j.jpba.2011.07.041. Epub 2011 Jul 30.
A simple and rapid isocratic, high performance liquid chromatography (HPLC) assay employing solid phase extraction (SPE) for the simultaneous determination of the anti HIV drug, efavirenz, the anti-tuberculosis drug, rifampicin and the desacetyl metabolite of rifampicin in plasma from HIV/tuberculosis infected patients has been developed. Using a Zorbax SB-Phenyl reverse-phase analytical column with UV detection, good separation and detection of the drugs was attained within a 10min run time. Intra- and inter-assay precision RSD values were found to be less than 15% at the concentrations examined (0.1-20μg/mL). The LOQ was found to be 0.1μg/mL for each agent and the assay was found to generate a linear response up to 20μg/mL. This low cost assay can accurately detect efavirenz and rifampicin concentrations within a clinically relevant concentration range using standard chromatography equipment, making it particularly applicable to resource-limited settings.
一种简单、快速的等度高效液相色谱（HPLC）分析方法，采用固相萃取（SPE），可同时测定 HIV/结核病感染患者血浆中的抗 HIV 药物依非韦伦、抗结核药物利福平以及利福平的去乙酰化代谢物。使用 Zorbax SB-Phenyl 反相分析柱和紫外检测，在 10 分钟的运行时间内可实现良好的药物分离和检测。在检测浓度范围内（0.1-20μg/mL），内、日间精密度 RSD 值均小于 15%。每个药物的 LOQ 均为 0.1μg/mL，该测定方法在 20μg/mL 浓度范围内呈线性响应。该低成本测定方法可使用标准色谱设备准确检测依非韦伦和利福平的浓度，适用于资源有限的环境。
