Ramachandran Geetha, Kumar A K Hemanth, Swaminathan Soumya, Venkatesan P, Kumaraswami V, Greenblatt David J
Tuberculosis Research Centre, Indian Council of Medical Research, Chetput, Chennai, India.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 1;835(1-2):131-5. doi: 10.1016/j.jchromb.2006.03.014. Epub 2006 Mar 29.
A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The method involved extraction of sample with ethyl acetate and analysis using a reversed-phase C(18) column (150 mm) with UV detection. The assay was linear from 0.0625 to 10.0 microg/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at -20 degrees C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz.
建立了一种简单快速的高效液相色谱法测定人血浆中的依非韦伦。该方法包括用乙酸乙酯萃取样品，并使用反相C(18)柱（150 mm）进行分析，采用紫外检测。该测定法在0.0625至10.0微克/毫升范围内呈线性。该方法对依非韦伦的测定具有特异性，且该药物在-20℃下于血浆中可稳定保存长达一个月。依非韦伦从血浆中的平均回收率为101%。由于其简便性，该测定法可用于依非韦伦的药代动力学研究和治疗药物监测。
