LC-MS/MS 法测定大鼠血浆中紫檀芪的浓度：药代动力学应用。
Determination of pinostrobin in rat plasma by LC-MS/MS: application to pharmacokinetics.
机构信息
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
出版信息
J Pharm Biomed Anal. 2011 Dec 5;56(4):841-5. doi: 10.1016/j.jpba.2011.07.038. Epub 2011 Jul 30.
A rapid and sensitive method for the determination of pinostrobin in rat plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS) for the first time. Isoliquiritigenin was used as an internal standard in rat plasma. Chromatographic separation was performed on an HiQ Sil C(18) column with isocratic elution at a flow rate of 1mL/min. The mobile phase consisted of water and methanol (9:91, v/v) containing 0.1% formic acid. The quantification limit was 10ng/mL within a linear range of 10-1000ng/mL (R=0.9984). The intra- and inter-day assay precision ranged from 3.8-5.3% to 3.2-5.2%, respectively, and the intra- and inter-day assay accuracy was between 93.2-95.1% and 95.5-104.3%, respectively. Our results indicated that the LC-MS/MS method is effective for pharmacokinetic study of pinostrobin in rat plasma.
首次建立了液相色谱-串联质谱法（LC-MS/MS）测定大鼠血浆中紫檀芪的方法。以异甘草素为内标，采用 HiQ Sil C18 柱，以水-甲醇（9:91，v/v）为流动相，含 0.1%甲酸，流速为 1mL/min，等度洗脱。紫檀芪在 10-1000ng/mL 范围内线性关系良好（R=0.9984），定量下限为 10ng/mL。日内和日间精密度分别为 3.8-5.3%至 3.2-5.2%，准确度分别为 93.2-95.1%至 95.5-104.3%。结果表明，该 LC-MS/MS 法可有效用于大鼠血浆中紫檀芪的药代动力学研究。
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