School of Physical and Mathematical Sciences and School of Biological Sciences, Nanyang Technological University, Singapore.
Nucleic Acids Res. 2011 Nov;39(21):9448-57. doi: 10.1093/nar/gkr539. Epub 2011 Aug 12.
G-rich oligonucleotides T30695 (or T30923), with the sequence of (GGGT)(4), and T40214, with the sequence of (GGGC)(4), have been reported to exhibit anti-HIV and anticancer activity. Here we report on the structure of a dimeric G-quadruplex adopted by a derivative of these sequences in K(+) solution. It comprises two identical propeller-type parallel-stranded G-quadruplex subunits each containing three G-tetrad layers that are stacked via the 5'-5' interface. We demonstrated control over the stacking of the two monomeric subunits by sequence modifications. Our analysis of possible structures at the stacking interface provides a general principle for stacking of G-quadruplexes, which could have implications for the assembly and recognition of higher-order G-quadruplex structures.
富含 G 的寡核苷酸 T30695（或 T30923），其序列为（GGGT）（4），以及 T40214，其序列为（GGGC）（4），据报道具有抗 HIV 和抗癌活性。在这里，我们报告了这些序列的衍生物在 K（+）溶液中形成的二聚体 G-四链体的结构。它由两个相同的螺旋桨型平行链 G-四链体亚基组成，每个亚基包含三个通过 5'-5' 界面堆叠的 G-四联体层。我们通过序列修饰证明了对两个单体亚基堆叠的控制。我们对堆叠界面上可能结构的分析为 G-四链体的堆叠提供了一个普遍原则，这可能对更高阶 G-四链体结构的组装和识别具有意义。
