Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
Hum Reprod. 2011 Oct;26(10):2869-73. doi: 10.1093/humrep/der267. Epub 2011 Aug 12.
Visfatin, which is secreted predominantly from visceral adipose tissue, has an insulin-mimetic action and may play a role in the regulation of insulin sensitivity in humans. Peripheral blood mononuclear cells (PBMCs) from venous blood samples are the most accessible tissue for the analysis of gene expression. The aims of the study were to compare the expression of visfatin in PBMCs with that in omental adipose tissue in women with polycystic ovary syndrome (PCOS).
Visfatin mRNA was measured in omental adipose tissue and PBMCs from 10 women with PCOS and 10 healthy controls, matched for BMI and age, using the real-time polymerase chain reaction (PCR).
The expression of visfatin mRNA in both omental adipose tissue and PBMCs from the women with PCOS was significantly higher (P = 0.01 and P = 0.05, respectively) than that in the controls. This finding indicated that mononuclear cells are a potential source of visfatin in women with PCOS. However, only the expression of visfatin mRNA in adipose tissue, not that in PBMCs, showed a significant positive correlation with insulin levels 2h after glucose loading (P = 0.044, r(2) = 0.45), and with homeostasis model assessment-insulin resistance (HOMA(IR); P = 0.035, r(2) = 0.47). In addition, the expression of visfatin mRNA in PBMCs did not correlate with the expression of visfatin mRNA in omental adipose tissue.
PCOS is associated with increased visfatin mRNA concentrations in PBMCs and in omental adipose tissue. However, only visfatin mRNA concentration in omental adipose tissue is closely correlated with BMI and insulin resistance.
内脏脂肪组织主要分泌的脂联素具有胰岛素模拟作用，可能在调节人体胰岛素敏感性方面发挥作用。外周血单核细胞（PBMC）是分析基因表达最容易获得的组织。本研究的目的是比较多囊卵巢综合征（PCOS）妇女 PBMC 中脂联素的表达与网膜脂肪组织中的表达。
使用实时聚合酶链反应（PCR）测量 10 例 PCOS 妇女和 10 例健康对照者的网膜脂肪组织和 PBMC 中脂联素 mRNA 的表达。
PCOS 妇女的网膜脂肪组织和 PBMC 中脂联素 mRNA 的表达均显著高于对照组（P = 0.01 和 P = 0.05）。这一发现表明，单核细胞是 PCOS 妇女脂联素的潜在来源。然而，只有脂肪组织中脂联素 mRNA 的表达，而不是 PBMC 中脂联素 mRNA 的表达，与葡萄糖负荷后 2 小时的胰岛素水平呈显著正相关（P = 0.044，r²=0.45），与稳态模型评估-胰岛素抵抗（HOMA（IR）；P = 0.035，r²=0.47）。此外，PBMC 中脂联素 mRNA 的表达与网膜脂肪组织中脂联素 mRNA 的表达无关。
PCOS 与 PBMC 和网膜脂肪组织中脂联素 mRNA 浓度增加有关。然而，只有网膜脂肪组织中脂联素 mRNA 浓度与 BMI 和胰岛素抵抗密切相关。
