Zhang Jing, Zhou Lingling, Tang Liulin, Xu Liangzhi
Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Department of Obstetrics and Gynecology, People's Hospital of Yicheng, Yicheng, Hubei 441400, P.R. China.
Exp Ther Med. 2014 May;7(5):1215-1220. doi: 10.3892/etm.2014.1556. Epub 2014 Feb 18.
Polycystic ovary syndrome (PCOS) is a common reproductive endocrinology disease, however, an explicit etiology is not known. Insulin resistance (IR) appears to be central to the pathogenesis of PCOS and inflammation may be significant in the pathogenesis of IR in PCOS. The aims of the present study were to investigate the plasma visfatin level and the gene expression of visfatin in peripheral blood mononuclear cells (PBMCs) and peripheral blood monocyte-derived macrophages (PBMMs) from PCOS patients, in addition to investigating the association between PCOS and IR. A total of 21 PCOS patients and 21 control subjects were enrolled in the study; the homeostasis model assessment of insulin resistance (HOMA-IR) was considered to be a stratified method for establishing the subgroups. Fasting blood samples were collected and the levels of sex hormones, insulin, glucose, blood lipids and visfatin were measured. In addition, visfatin gene expression levels in PBMCs and PBMMs were assessed using quantitative polymerase chain reaction. The plasma visfatin and gene expression levels of visfatin in PBMCs and PBMMs were not observed to increase in the normal weight PCOS and normal weight IR patients. Furthermore, plasma visfatin levels did not correlate with the normal weight PCOS patients or the normal weight IR patients . Further investigation into the role of visfatin in the pathogenesis of PCOS or IR should examine macrophages in the tissues, rather than macrophages in the peripheral blood.
多囊卵巢综合征（PCOS）是一种常见的生殖内分泌疾病，然而，其确切病因尚不清楚。胰岛素抵抗（IR）似乎是PCOS发病机制的核心，炎症在PCOS的IR发病机制中可能起重要作用。本研究的目的是调查PCOS患者外周血单核细胞（PBMC）和外周血单核细胞衍生巨噬细胞（PBMM）中内脂素的血浆水平和基因表达，此外还调查PCOS与IR之间的关联。共有21例PCOS患者和21例对照受试者纳入本研究；胰岛素抵抗的稳态模型评估（HOMA-IR）被认为是建立亚组的分层方法。采集空腹血样，检测性激素、胰岛素、葡萄糖、血脂和内脂素水平。此外，使用定量聚合酶链反应评估PBMC和PBMM中内脂素基因表达水平。在正常体重的PCOS患者和正常体重的IR患者中，未观察到PBMC和PBMM中血浆内脂素及其基因表达水平升高。此外，血浆内脂素水平与正常体重的PCOS患者或正常体重的IR患者均无相关性。对内脂素在PCOS或IR发病机制中的作用进行进一步研究时，应检查组织中的巨噬细胞，而非外周血中的巨噬细胞。
