Lohuis J A, Schukken Y H, Henricks P A, Heyneman R, Burvenich C, Verheijden J H, Van Miert A S, Brand A
College of Veterinary Medicine, State University of Utrecht, The Netherlands.
J Dairy Sci. 1990 Feb;73(2):342-50. doi: 10.3168/jds.S0022-0302(90)78679-1.
The relationship between preinfection functions of blood neutrophils and outcome of experimental Escherichia coli mastitis was studied in 11 cows. Random migration, chemotaxis, phagocytosis, and chemiluminescence by neutrophils were determined in white blood cell suspensions, and in purified neutrophil suspensions. The course of E. coli mastitis (10(4) E. coli 0:157 in rear quarters) was monitored using clinical parameters, counts of E. coli in mastitic secretion, and milk production. Regressions were calculated for areas under curves of these parameters and preinfection activities of neutrophils. Chemiluminescence by nonstimulated neutrophils in white blood cell suspensions was negatively correlated with counts of E. coli in secretion and with losses in milk production. The chemotactic differential in white blood cell suspensions minus the chemotactic differential in purified suspensions of neutrophils referred to as delta varied from -.66 to +.50, indicating, respectively, inhibition and stimulation of chemotactic activity of neutrophils in white blood cell suspensions. Delta correlated negatively with counts of E. coli in mastitis secretion, inhibition of the amplitude of rumen contractions, and losses in milk production. We hypothesize that a factor in white blood cell suspensions may be involved in the down-regulation of the migratory response of neutrophils during E. coli mastitis.
在11头奶牛中研究了血液中性粒细胞感染前功能与实验性大肠杆菌乳腺炎结果之间的关系。在白细胞悬液和纯化的中性粒细胞悬液中测定中性粒细胞的随机迁移、趋化性、吞噬作用和化学发光。使用临床参数、乳腺炎分泌物中大肠杆菌的计数和产奶量来监测大肠杆菌乳腺炎（后乳房注入10⁴个大肠杆菌O:157）的病程。计算这些参数曲线下面积与中性粒细胞感染前活性之间的回归关系。白细胞悬液中未受刺激的中性粒细胞的化学发光与分泌物中大肠杆菌的计数以及产奶量损失呈负相关。白细胞悬液中的趋化差异减去中性粒细胞纯化悬液中的趋化差异（称为δ）在-.66至+.50之间变化，分别表明白细胞悬液中中性粒细胞趋化活性受到抑制和刺激。δ与乳腺炎分泌物中大肠杆菌的计数、瘤胃收缩幅度的抑制以及产奶量损失呈负相关。我们推测白细胞悬液中的一个因素可能参与了大肠杆菌乳腺炎期间中性粒细胞迁移反应的下调。
