Kremer W D, Noordhuizen-Stassen E N, Grommers F J, Daemen A J, Henricks P A, Brand A, Burvenich C
University of Utecht, Department of Herd Health and Reproduction, The Netherlands.
J Dairy Sci. 1993 Jun;76(6):1568-74. doi: 10.3168/jds.S0022-0302(93)77490-1.
Experimental mastitis was induced by inoculating rear right quarters of 10 healthy cows with 10(3) cfu of Escherichia coli. The chemotactic responses of peripheral blood polymorphonuclear leukocytes at d-6, -5, -2, -1, and immediately prior to inoculation were measured. Chemiluminescence of polymorphonuclear leukocytes was measured immediately prior to inoculation. Severity of the experimental mastitis was assessed by bacterial growth in the inoculated quarters. Results of this study indicated that severity of the experimental mastitis may be predicted by the chemotactic response in vitro of polymorphonuclear leukocytes isolated from the peripheral blood at d 2, d 1, and immediately prior to inoculation. The number of circulating polymorphonuclear leukocytes immediately prior to inoculation also showed a negative relationship with the severity of mastitis. No relationship existed between preinfection chemiluminescence of polymorphonuclear leukocytes and the severity of the experimental mastitis.
通过向10头健康奶牛的右后乳房接种10³cfu的大肠杆菌诱导实验性乳腺炎。测量了接种前第6天、第5天、第2天、第1天以及即将接种前外周血多形核白细胞的趋化反应。在即将接种前测量多形核白细胞的化学发光。通过接种乳房中的细菌生长评估实验性乳腺炎的严重程度。本研究结果表明，实验性乳腺炎的严重程度可通过在接种前第2天、第1天以及即将接种前从外周血中分离的多形核白细胞的体外趋化反应来预测。接种前即刻循环多形核白细胞的数量也与乳腺炎的严重程度呈负相关。多形核白细胞感染前的化学发光与实验性乳腺炎的严重程度之间不存在相关性。
