Heine H, Schaeg G
Acta Anat (Basel). 1979;103(1):1-10. doi: 10.1159/000144992.
Both organ culture (choriallantoic membrane) of striped muscle from mouse diaphragm and freeze-drying of myocardial cells presented very characteristic structures in mitochondria. The organ culture evidence 'rod-like structures' in the intracristal spaces of the cristae mitochondriales. It is believed that these structures are the central apparatus responsible for mitochondrial division. On the other hand, the freeze-dried mitochondria presented neither intracristal spaces nor a space in the outer mitochondrial membrane. Because these spaces appeared only after fixation and dehydration, it is discussed if they are really artifacts. The mitochondrial cristae appearing as united structures after the freeze-drying process have been compared to the rod-like structures appearing intracristally after organ culture and conventional preparation techniques.
来自小鼠膈肌的横纹肌组织培养（绒毛尿囊膜）和心肌细胞冻干处理后的线粒体均呈现出非常独特的结构。组织培养显示线粒体嵴的嵴内间隙中有“杆状结构”。据信这些结构是负责线粒体分裂的核心装置。另一方面，冻干后的线粒体既没有嵴内间隙，线粒体外膜也没有间隙。由于这些间隙仅在固定和脱水后才出现，因此讨论了它们是否真的是人为假象。将冻干处理后呈现为联合结构的线粒体嵴与组织培养及传统制备技术后嵴内出现的杆状结构进行了比较。
